Organic/inorganic double-layered shells for multiple cytoprotection of individual living cells

The cytoprotection of individual living cells under and daily-life conditions is a prerequisite for various cell-based applications including cell therapy, cell-based sensors, regenerative medicine, and even the food industry. In this work, we use a cytocompatible two-step process to encapsulate in...

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Published inChemical science (Cambridge) Vol. 6; no. 1; pp. 203 - 208
Main Authors Hong, Daewha, Lee, Hojae, Ko, Eun Hyea, Lee, Juno, Cho, Hyeoncheol, Park, Matthew, Yang, Sung Ho, Choi, Insung S
Format Journal Article
LanguageEnglish
Published England 01.01.2015
Subjects
Biochemistry
Cells (biology)
Cellular
Encapsulation
Enzymes
Filtering
Nanostructure
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Summary:The cytoprotection of individual living cells under and daily-life conditions is a prerequisite for various cell-based applications including cell therapy, cell-based sensors, regenerative medicine, and even the food industry. In this work, we use a cytocompatible two-step process to encapsulate in a highly uniform nanometric (<100 nm) shell composed of organic poly(norepinephrine) and inorganic silica layers. The resulting cell-in-shell structure acquires multiple resistance against lytic enzyme, desiccation, and UV-C irradiation. In addition to the UV-C filtering effect of the double-layered shell, the biochemical responses of the encapsulated yeast are suggested to contribute to the observed UV-C tolerance. This work offers a chemical tool for cytoprotecting individual living cells under multiple stresses and also for studying biochemical behavior at the cellular level.
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ISSN:2041-6520
2041-6539
DOI:10.1039/c4sc02789b