Optimizing PCL/PLGA Scaffold Biocompatibility Using Gelatin from Bovine, Porcine, and Fish Origin

This research introduces a novel approach by incorporating various types of gelatins, including bovine, porcine, and fish skin, into polycaprolactone and poly (lactic-co-glycolic acid) using a solvent casting method. The films are evaluated for morphology, mechanical properties, thermal stability, b...

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Published inGels Vol. 9; no. 11; p. 900
Main Authors Ghafouri Azar, Mina, Wiesnerova, Lucie, Dvorakova, Jana, Chocholata, Petra, Moztarzadeh, Omid, Dejmek, Jiri, Babuska, Vaclav
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.11.2023
Subjects
Amino acids
Biocompatibility
Cartilage
Cattle
Cell adhesion
Cell adhesion & migration
cell viability
Composite materials
Drug delivery systems
Elongation
Fish skins
Flexibility
Founding
Gelatin
Glycolic acid
Mechanical properties
Methods
Morphology
Polycaprolactone
Polymers
Skin
Stability analysis
Stress-strain curves
Tensile strength
Thermal stability
Tissue engineering
Toxicity
Weight loss
Czech Republic
Germany
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Summary:This research introduces a novel approach by incorporating various types of gelatins, including bovine, porcine, and fish skin, into polycaprolactone and poly (lactic-co-glycolic acid) using a solvent casting method. The films are evaluated for morphology, mechanical properties, thermal stability, biodegradability, hemocompatibility, cell adhesion, proliferation, and cytotoxicity. The results show that the incorporation of gelatins into the films alters their mechanical properties, with a decrease in tensile strength but an increase in elongation at break. This indicates that the films become more flexible with the addition of gelatin. Gelatin incorporation has a limited effect on the thermal stability of the films. The composites with the gelatin show higher biodegradability with the highest weight loss in the case of fish gelatin. The films exhibit high hemocompatibility with minimal hemolysis observed. The gelatin has a dynamic effect on cell behavior and promotes long-term cell proliferation. In addition, all composite films reveal exceptionally low levels of cytotoxicity. The combination of the evaluated parameters shows the appropriate level of biocompatibility for gelatin-based samples. These findings provide valuable insights for future studies involving gelatin incorporation in tissue engineering applications.
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ISSN:2310-2861
2310-2861
DOI:10.3390/gels9110900