ZnO nanoparticles effect on pollen grain germination and pollen tube elongation

• Published in: Plant cell, tissue and organ culture Vol. 145; no. 2; pp. 405 - 415
• Main Authors: Yoshihara, Shizue, Hirata, Saki, Yamamoto, Kasumi, Nakajima, Yoshino, Kurahashi, Kensuke, Tokumoto, Hayato
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2021; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Calcium chloride; Calcium ions; Elongation; Exposure; Germ cells; Germination; Life Sciences; Moisture content; Nanoparticles; Original Article; Plant cells; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Pollen; Pollen tubes; Solubility; Water content; Zinc chloride; Zinc oxide; Zinc oxides; Metal oxide nanoparticles; ZnO; Zn; Pollen germination
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-021-02017-2

Plant germ cells, such as pollen grains, can be affected by exposure to metal nanoparticles (NPs) that have diffused into the environment. The germination and tube elongation of pollen grain ( Lilium longiflorum ) exposed to low-solubility NPs was observed. The germination rate of pollen grain exposed to 100 mg L −1 ZnO NP dispersion decreased significantly from controls and exposure of the other NPs. On the other hand, when pollens were exposed to ionic solutions in which particles were removed from the 100 mg L −1 ZnO NP dispersion, the germination rates were equivalent to those in the control pollens. On the contrary, decrease of the germination rate compared to controls was small in the exposure to ZnCl 2 solution, which contained a larger amount of water-soluble Zn 2+ than ZnO NP dispersion. From these results, it was concluded that fine ZnO NP may adhere to pollens, due to the cohesive property of nanoparticles, and Zn 2+ dissolved at the interface may be continuously absorbed by pollens. When pollen was exposed to ZnO NP, a spot with a high Ca 2+ local concentration was observed at the tip of the pollen tube. On the contrary, simultaneous exposure to antagonistic ZnO NP and CaCl 2 resulted in no decrease in the germination rate. From the above, it is considered that upon exposure to ZnO NP, cells absorb Zn 2+ depending on the specific solubility of ZnO NP. Key Message Despite the low solubility of zinc oxide nanoparticle, pollen cell-attached particles inhibited germination and elongation of pollen tube by continuous Zn 2+ dissolution from particles and Zn 2+ absorption by the cell.