Effects of Aflatoxin B1 and Fumonisin B1 on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes

The present study evaluated the effect of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel e...

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Bibliographic Details
Published inInternational journal of molecular sciences Vol. 11; no. 4; pp. 1944 - 1955
Main Authors Ribeiro, Deise H. B., Ferreira, Fabiane L., Da Silva, Valéria N., Aquino, Simone, Corrêa, Benedito
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.04.2010
Molecular Diversity Preservation International (MDPI)
Subjects
aflatoxin B1
Apoptosis
Cell culture
Flow cytometry
fumonisin B1
Kinases
Metabolites
Morphology
primary hepatocytes
Toxicity
Brazil
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Summary:The present study evaluated the effect of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB1 and FB1 used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms11041944