Determination of efficiency of attachment of biotinylated antibodies to avidin-linked, aqueous-based microcapsules by flow cytometry

We used flow cytometry to determine the percentage of aqueous-based microcapsules bearing antibodies specific for various antigen-presenting cells (APCs) within a given population of putative APC-specific microcapsules. Flow cytometry offers a high-throughput, rapid and simple method to analyze anti...

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Bibliographic Details
Published inJournal of immunological methods Vol. 277; no. 1; pp. 135 - 139
Main Authors Chow, Andrea A., Moser, Charlotte A., Speaker, Tully J., Offit, Paul A.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.06.2003
Elsevier
Subjects
Alginate
Animals
Antibodies - metabolism
Antigen-Presenting Cells - metabolism
Aqueous-based microcapsules
Avidin
Avidin - metabolism
Biological and medical sciences
Biotin - metabolism
Biotinylated antibody
Capsules
Flow cytometry
Flow Cytometry - methods
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Molecular immunology
Spermine
Techniques
Biotinylated antibody
Flow cytometry
APCs
Aqueous-based microcapsules
KLH
Avidin
DMEM
FITC
Spermine
MC
RRV
Alginate
dH 2O
iRRV
Antigen presentation
Antibody
Microcapsule
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Summary:We used flow cytometry to determine the percentage of aqueous-based microcapsules bearing antibodies specific for various antigen-presenting cells (APCs) within a given population of putative APC-specific microcapsules. Flow cytometry offers a high-throughput, rapid and simple method to analyze antibody binding to noncellular, nonspherical material.
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ISSN:0022-1759
1872-7905
DOI:10.1016/S0022-1759(03)00175-3