Simple and rapid method determination for metformin in human plasma using high performance liquid chromatography tandem mass spectrometry: Application to pharmacokinetic studies

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 852; no. 1; pp. 308 - 316
• Main Authors: Marques, Marlice A. Sípoli, Soares, Alcenir de Souza, Pinto, Olivia Woyames, Barroso, Pedro Tupinambá Werneck, Pinto, Douglas Pereira, Ferreira-Filho, Milton, Werneck-Barroso, Eduardo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.2007; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Calibration; Chromatography, High Pressure Liquid - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; Humans; Hypoglycemic Agents - blood; Hypoglycemic Agents - pharmacokinetics; Liquid chromatography; Mass spectrometry; Medical sciences; Metformin; Metformin - blood; Metformin - pharmacokinetics; Pharmacokinetic; Pharmacology. Drug treatments; Plasma; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods; Plasma; Liquid chromatography; Metformin; Mass spectrometry; Pharmacokinetic; Human; Biological fluid; HPLC chromatography; Determination; Blood plasma; Hypoglycemic agent; Rapid technique; Biguanides; Pharmacokinetics; Quantitative analysis

A rapid and simple method for quantitation of metformin (MET) in human plasma by HPLC–MS/MS was developed and validated. The sample preparation consists of plasma deproteinization using acetonitrile. The mobile phase consisted of water–acetonitrile and formic acid (55/45/0.048, v/v/%) and the run ti...