Simple and rapid method determination for metformin in human plasma using high performance liquid chromatography tandem mass spectrometry: Application to pharmacokinetic studies

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 852; no. 1; pp. 308 - 316
• Main Authors: Marques, Marlice A. Sípoli, Soares, Alcenir de Souza, Pinto, Olivia Woyames, Barroso, Pedro Tupinambá Werneck, Pinto, Douglas Pereira, Ferreira-Filho, Milton, Werneck-Barroso, Eduardo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.2007; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Calibration; Chromatography, High Pressure Liquid - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; Humans; Hypoglycemic Agents - blood; Hypoglycemic Agents - pharmacokinetics; Liquid chromatography; Mass spectrometry; Medical sciences; Metformin; Metformin - blood; Metformin - pharmacokinetics; Pharmacokinetic; Pharmacology. Drug treatments; Plasma; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods; Plasma; Liquid chromatography; Metformin; Mass spectrometry; Pharmacokinetic; Human; Biological fluid; HPLC chromatography; Determination; Blood plasma; Hypoglycemic agent; Rapid technique; Biguanides; Pharmacokinetics; Quantitative analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2007.01.030

A rapid and simple method for quantitation of metformin (MET) in human plasma by HPLC–MS/MS was developed and validated. The sample preparation consists of plasma deproteinization using acetonitrile. The mobile phase consisted of water–acetonitrile and formic acid (55/45/0.048, v/v/%) and the run time was 3 min. A pursuit C 18 (100 mm × 2.0 mm i.d., 3 μm) column connected to a guard column MS-pursuit (0.20 mm × 0.20 mm i.d., 5 μm) was used. The range of the calibration curve was from 20 to 5000 ng/mL, the limit of quantitation being 20 ng/mL. The detection was performed on a mass spectrometer (ESI +), using metoprolol as internal standard. The calibration curves have r 2 values of 0.995 (CV = 0.24%, n = 10). The accuracy and precision were between 90.74 and 106.7% and coefficients of variations (CV) of 1.10 and 4.35%, respectively. The method was applied to determine the pharmacokinetic parameters: C max (1667.25 ng/mL) and T max (3.89 h).