Sequential dual site-selective protein labelling enabled by lysine modification

[Display omitted] Methods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of-concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically enc...

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Bibliographic Details
Published inBioorganic & medicinal chemistry Vol. 28; no. 22; p. 115783
Main Authors Matos, Maria J., Brown, Libby, Bernardim, Barbara, Guerreiro, Ana, Jiménez-Osés, Gonzalo, Bernardes, Gonçalo J.L.
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 15.11.2020
Elsevier
Subjects
Albumin
Antibodies
Bioorthogonal labelling
Lysine modification
Protein modification
Albumin
Antibodies
Lysine modification
Bioorthogonal labelling
Protein modification
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Summary:[Display omitted] Methods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of-concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically encoded ketone-tagged amino acids. This sequential, orthogonal approach was applied to albumin and a therapeutic antibody to create functional dual site-selectively labelled proteins.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0968-0896
1464-3391
DOI:10.1016/j.bmc.2020.115783