Liquid chromatographic–fluorimetric method for the estimation of nitric oxide biosynthesis in the central nervous system

Some amino acids are involved in the biosynthesis of nitric oxide (NO), which has a physiological and pathophysiological role. To study NO biosynthesis, we compared arginine and citrulline levels in the cerebrospinal fluid (CSF) from patients with infectious and/or inflammatory processes within the...

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Published inJournal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 806; no. 2; pp. 133 - 139
Main Authors Pérez-Neri, Iván, Montes, Sergio, Boll, Marie-Catherine, Ramı́rez-Bermúdez, Jesús, Rı́os, Camilo
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 05.07.2004
Elsevier Science
Subjects
Adult
Analysis
Analytical, structural and metabolic biochemistry
Arginine
Biological and medical sciences
Central Nervous System - metabolism
Chromatography, Liquid - methods
Citrulline
Fluorometry - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
Medical sciences
Nitric oxide
Nitric Oxide - biosynthesis
Pharmacology. Drug treatments
Reproducibility of Results
Sensitivity and Specificity
Arginine
Nitric oxide
Citrulline
Human
Nervous system diseases
Hepatoprotector
Central nervous system
Parkinson disease
Schizophrenia
Biosynthesis
Liquid chromatography
Cerebrospinal fluid
Fluorescence spectrometry
Psychosis
Aminoacid
Degenerative disease
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Summary:Some amino acids are involved in the biosynthesis of nitric oxide (NO), which has a physiological and pathophysiological role. To study NO biosynthesis, we compared arginine and citrulline levels in the cerebrospinal fluid (CSF) from patients with infectious and/or inflammatory processes within the central nervous system (CNS), with those from patients without those disorders. Arginine concentration was not significantly different between the groups ( P=0.115), whereas citrulline was significantly elevated in the first group ( P=0.020). We propose a simple chromatographic method to estimate NO biosynthesis ex vivo within the CNS, that may be applicable for the study of neurodegenerative and psychiatric diseases such as Parkinson’s disease and schizophrenia.
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ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2004.03.043