Implication of microRNAs as messengers of exercise adaptation in junior female triathlonists

• Published in: Scientific reports Vol. 14; no. 1; pp. 22858 - 12
• Main Authors: Gaál, Zsuzsanna, Fodor, János, Oláh, Tamás, Szabó, Ivett Gabriella, Balatoni, Ildikó, Csernoch, László
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/208/176; 631/337/384/331; 631/443; Adaptation; Adaptation, Physiological - genetics; Adolescent; Adult; Athletes; Bicycling - physiology; Blood; Exercise - physiology; Female; Females; Humanities and Social Sciences; Humans; Metabolism; MicroRNAs; MicroRNAs - blood; MicroRNAs - genetics; miRNA; multidisciplinary; Physical Endurance; Running - physiology; Science; Science (multidisciplinary); Young Adult
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-73670-8

While expression profile of muscle-specific miRNAs following endurance training is well-characterized, information about exercise-induced changes of metabolism-regulating miRNAs is limited, especially in female and junior athletes. Major aim of this study was to examine a set of miRNAs related to mitochondrial function and metabolism in highly professional junior female athletes. The Hungarian National Junior Triathlon Team (n = 4), completed standardized running and cycling sessions. Expression levels of miR-133a, miR-210, miR-494 and miR-127-3p were determined by RT-qPCR in whole blood and serum samples, withdrawn directly before, and after the exercise, and 24 and 48 h later. The expression of miR-494, miR-127-3p and miR-210 showed strong correlation with each other. In serum, nearly significant increment of miR-127-3p levels was detected, that may be a novel biomarker of exercise adaptation. Its expression was significantly higher than that of miR-210. In whole blood, significantly higher miR-210 than miR-494 and miR-127-3p levels were observed. MiRNA expression profile of the youngest athlete was markedly different compared to others. Our results suggest that miRNAs related to mitochondrial function and metabolism are involved in exercise adaptation. The present study may facilitate further research with larger potential participant pools, contributing to improved prevention and treatment of chronic diseases of civilization.