Elevated α-esterase levels associated with permethrin tolerance in Aedes aegypti (L.) from Baja California, Mexico

• Published in: Pesticide biochemistry and physiology Vol. 82; no. 1; pp. 66 - 78
• Main Authors: Flores, Adriana E., Albeldaño-Vázquez, Walter, Salas, Ildefonso Fernandez, Badii, Mohammad H., Becerra, Haydeé Loaiza, Garcia, Gustavo Ponce, Fuentes, Saúl Lozano, Brogdon, William G., Black IV, William C., Beaty, Barry
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.05.2005; Elsevier
• Subjects: Aedes aegypti; Biological and medical sciences; Control; Esterases; Fundamental and applied biological sciences. Psychology; insecticide resistance; Medically important nuisances and vectors, pests of stored products and materials: population survey and control; Permethrin; Phytopathology. Animal pests. Plant and forest protection; Protozoa. Invertebrates; Resistance; Vectors. Intermediate hosts; USA, Louisiana, New Orleans; Resistance; Aedes aegypti; Esterases; Permethrin; Insecticide; Enzyme; Insecta; Pesticides; Pyrethroids; Arthropoda; Culicidae; Hydrolases; Invertebrata; Diptera
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2004.12.007

A discriminating dose for permethrin for Aedes aegypti from Baja California, Mexico was determined, and was used to select individuals. Mosquitoes were collected from four different municipalities located in the north and south end of the Baja California Peninsula. Individuals were chosen for further study based on their similar response to the insecticide. We exposed 10 groups of 90 Ae. aegypti females to the discriminating dose (172 μg/ml) and after producing 50% mortality, individuals were divided into two categories: killed and survivors. Each of these groups was dissected to separate the head, thorax, and abdomen. Biochemical tests were performed on the head and thorax to determine resistance-related enzyme activities including: α- and β-esterases, glutathione S-transferase, acetyl cholinesterase, insensitive acetyl cholinesterase, and mixed-function oxidases. The results were compared with those for the susceptible New Orleans strain of Ae. aegypti. All the populations studied showed the consistent presence of α-esterases, with elevated levels in permethrin-selected populations. Although β-esterases and GST levels were present in high proportions, they did not reveal a clear pattern in relation to resistance.