Virtues and limitations of Pittsburgh green for ozone detection

A recently proposed 2',7'-dichlorofluorescein (DCF)-derived fluorescent probe for the detection of ozone shows good selectivity against a number of reactive oxygen species and good pH stability for biological and environmental applications. It is found, however, that over oxidation of the...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 51; no. 9; pp. 1579 - 1582
Main Authors Castelló Beltrán, Carlos, Palmer, Elliott A, Buckley, Benjamin R, Iza, Felipe
Format Journal Article
LanguageEnglish
Published England 01.01.2015
Subjects
Byproducts
Chemistry Techniques, Analytical - standards
Design analysis
Drug Stability
Fluorescence
Fluorescent Dyes - chemistry
Hydrogen-Ion Concentration
Molecular Structure
Monitoring
NMR spectroscopy
Oxidation
Ozone
Ozone - analysis
Stability
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Summary:A recently proposed 2',7'-dichlorofluorescein (DCF)-derived fluorescent probe for the detection of ozone shows good selectivity against a number of reactive oxygen species and good pH stability for biological and environmental applications. It is found, however, that over oxidation of the fluorescent product (Pittsburgh green) can occur. This could render quantitative measurements inaccurate due to a reduction in fluorescence and overlapping fluorescence signals from over oxidation by-products and it requires careful experimental design. Although difficult to assess by fluorescence measurements, the over oxidation can be conveniently monitored by (1)H NMR spectroscopy.
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ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc08244c