Simultaneous quantitation of cytokeratin-19 fragment and carcinoembryonic antigen in human serum via quantum dot-doped nanoparticles

• Published in: Biosensors & bioelectronics Vol. 91; pp. 60 - 65
• Main Authors: Chen, Zhenhua, Liang, Rongliang, Guo, Xinxin, Liang, Junyu, Deng, Qiaoting, Li, Min, An, Taixue, Liu, Tiancai, Wu, Yingsong
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.05.2017
• Subjects: Antibodies, Immobilized - chemistry; Antigens, Neoplasm - blood; Biomarkers, Tumor - blood; Biosensing Techniques - instrumentation; Cadmium Compounds - chemistry; Carcinoembryonic Antigen - blood; CEA; CYFRA 21-1; Equipment Design; Fluorescent Dyes - chemistry; Humans; Keratin-19 - blood; Lateral flow test strips; Limit of Detection; Lung Neoplasms - blood; Lung Neoplasms - diagnosis; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Polystyrene nanoparticle; Polystyrenes - chemistry; Prognosis; Quantum dots; Quantum Dots - chemistry; Quantum Dots - ultrastructure; Reagent Strips - analysis; Reproducibility of Results; Simultaneous quantitation; Sulfides - chemistry; Tellurium - chemistry; Polystyrene nanoparticle; Quantum dots; CYFRA 21-1; CEA; Lateral flow test strips; Simultaneous quantitation
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2016.12.036

A novel quantum dot-doped polystyrene nanoparticles-based lateral flow test strips (QPs-LFTS) system was developed to simultaneously detect a cytokeratin-19 fragment (CYFRA 21-1) and carcinoembryonic antigen (CEA) in human serum to aid the diagnosis and prognosis of lung cancer. Quantum dot-doped carboxylate-functionalized polystyrene nanoparticles (QPs) were prepared and introduced as fluorescent reporters in QPs-LFTS. The detection was based on a sandwich immunoassay and performed on lateral flow test strips, with an assay time of 15min. The strips were read by a fluorescence strip reader to obtain the fluorescence peak heights of the test lines (HT) and the control line (HC). The ratio of HT/HC was used for quantitation. The QPs showed excellent photoproperties and good performance. Under optimal conditions, the QPs-LFTS system exhibited a wide linear range for CYFRA 21-1 (1.3–480ng/mL) and CEA (2.8–680ng/mL). The detection limits for CYFRA 21-1 and CEA were 0.16 and 0.35ng/mL, respectively. The recovery and reproducibility of the method were satisfactory. Furthermore, excellent correlations (n =120, R2 =0.9862, P<0.0001 for CYFRA 21-1; n =70, R2 =0.9509, P<0.0001 for CEA) were obtained between the QPs-LFTS and commercially available chemiluminescence immunoassay kits in clinical serum testing. The results indicate that this developed test system is highly efficient and is expected to be useful for early screening and prognosis evaluation for lung cancer patients. •A multiplex lateral flow test strips for simultaneous detection of CYFRA 21-1 and CEA has been developed.•CdTe/CdSe hydrophobic quantum dots were synthesized and were used in dyeing polystyrene microparticles.•The HT/HC ratio was used to quantify CYFRA 21-1 and CEA in samples.•The limit of detection was 160pg/mL for CYFRA 21-1 and 350pg/mL for CEA.