Intramammary infections with different non-aureus staphylococci in dairy cows

• Published in: Journal of dairy science Vol. 101; no. 2; pp. 1403 - 1418
• Main Authors: Nyman, A.-K., Fasth, C., Waller, K. Persson
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2018
• Subjects: Animal and Dairy Science; Animals; antimicrobial susceptibility; beta-Lactamases - biosynthesis; California; Cattle; Cell Count - veterinary; coagulase-negative staphylococci; Dairying; Female; Husdjursvetenskap; mastitis; Mastitis, Bovine - microbiology; Milk - cytology; Parity; persistence; Pregnancy; Staphylococcus - classification; Staphylococcus - isolation & purification; Staphylococcus epidermidis - isolation & purification; Staphylococcus haemolyticus - isolation & purification; Staphylococcus hyicus - isolation & purification; Sweden; antimicrobial susceptibility; persistence; mastitis; coagulase-negative staphylococci
• ISSN: 0022-0302; 1525-3198; 1525-3198
• DOI: 10.3168/jds.2017-13467

Subclinical mastitis causes an increase in milk somatic cell count (SCC) and can lead to reduced milk production and early culling. In many countries, non-aureus staphylococci (NAS) is the most common bacterial finding in subclinical mastitis of dairy cows. New methodology makes it possible to identify NAS species, but knowledge about the epidemiology is limited. The objective of this project was to improve advisory services for mastitis control by investigating associations between NAS and SCC, milk production, and persistence of intramammary infections (IMI). Farmers who had sent milk samples to the Swedish National Veterinary Institute (Uppsala, Sweden) were asked to participate if NAS was identified in the samples. Participating farmers were asked to resample all udder quarters of the cow once within 1 mo. Regression models were used to investigate associations between NAS and cow factors, udder quarter California mastitis test and SCC, and persistence of IMI. Associations with cow composite milk yield and SCC were also investigated. In total, 671 cows from 201 herds were enrolled in the study, and 19 NAS species were identified, of which the 4 most common were Staphylococcus epidermidis, Staphylococcus simulans, Staphylococcus chromogenes, and Staphylococcus haemolyticus. Persistent IMI was more common in udder quarters with Staphylococcus hyicus and S. simulans and less common in those with Staphylococcus saprophyticus IMI. β-Lactamase production by the different NAS species varied from 0 to 100%. There was a significant association between NAS species and California mastitis test and SCC of udder quarters, and this varied depending on parity. The cow composite milk SCC at the test milking before the initial sample was taken differed significantly with NAS species, but not at the subsequent test milking. Milk yield—at the test milking before or after the initial sample—did not differ significantly for NAS species. There were no significant associations between milk yield or SCC and persistent NAS IMI. In conclusion, the NAS species affects SCC and persistent IMI differently but not milk yield.