Podocarpane, isopimarane, and abietane diterpenoids from Isodon lophanthoides var. graciliflorus

• Published in: Food chemistry Vol. 136; no. 3-4; pp. 1177 - 1182
• Main Authors: Liang, Yaoguang, Xie, Haihui, Wu, Ping, Jiang, Yueming, Wei, Xiaoyi
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.02.2013; Elsevier
• Subjects: Abietane; aerial parts; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - pharmacology; Biological and medical sciences; carcinoma; Cell Line, Tumor; Cytotoxicity; Diterpenes - chemistry; Diterpenes - pharmacology; Diterpenes, Abietane - chemistry; Diterpenes, Abietane - pharmacology; diterpenoids; Food industries; Fundamental and applied biological sciences. Psychology; Humans; Isodon - chemistry; Isodon lophanthoides; Isodon lophanthoides var. graciliflorus; Isopimarane acetal; Plant Extracts - chemistry; Plant Extracts - pharmacology; Podocarpane; Abietane; Cytotoxicity; Podocarpane; Isopimarane acetal; Isodon lophanthoides var. graciliflorus; Acetal; Terpenoid
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2012.09.084

► Four new diterpenoids isolated from Isodon lophanthoides var. graciliflorus. ► Graciliflorin D possessed a rearranged abietane skeleton. ► Cytotoxicity evaluated against three human carcinoma cell lines. ► Micranthin B showed in vitro cytotoxicity against all the tested cell lines. Four new diterpenoids including two podocarpanes, graciliflorins A (1) and B (2), an isopimarane acetal, graciliflorin C (3), and a rearranged abietane, graciliflorin D (4) were isolated from the aerial parts of Isodon lophanthoides var. graciliflorus (Lamiaceae) along with podocarpa-8,11,13-triene-3α,13-diol (5) and micranthin B (6). Their structures were elucidated based on the spectroscopic data. The in vitro cytotoxicity of compounds 1–4 and 6 against human carcinoma A549, MCF-7, and HeLa cell lines were evaluated using the MTT colourimetric assay. Micranthin B (6) showed moderate activity against all the cells with IC50 values of 16.29, 18.20, and 22.25μM, while compounds 1–4 were inactive (IC50>50μg/ml).