Multicenter evaluation of the novel enzyme immunoassay based on P1-enriched protein for the detection of Mycoplasma pneumoniae infection

• Published in: Journal of microbiological methods Vol. 47; no. 1; pp. 65 - 71
• Main Authors: Suni, Jukka, Vainionpää, Raija, Tuuminen, Tamara
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Shannon Elsevier B.V 01.10.2001; Elsevier Science
• Subjects: Adhesins, Bacterial - immunology; Adult; Antibodies, Bacterial - blood; Bacterial diseases; Bacteriology; Biological and medical sciences; EIA; Enzyme immunoassay; Experimental bacterial diseases and models; Female; Fundamental and applied biological sciences. Psychology; Humans; Immunoenzyme Techniques; Infectious diseases; Male; Medical sciences; Microbiology; Mycoplasma pneumoniae; Mycoplasma pneumoniae - immunology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Pneumonia, Mycoplasma - diagnosis; Reagent Kits, Diagnostic; Sensitivity and Specificity; Serodiagnosis; Europe; Finland; Mycoplasma pneumoniae; Serodiagnosis; Enzyme immunoassay; EIA; Enrichment; Evaluation; IgA; Enzyme; Acute; IgG; Mycoplasmataceae; IgM; Protein; Mollicutes; Infection; Mycoplasmatales; Bacteria; Detection
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/S0167-7012(01)00291-3

The aim of the study was to evaluate new Mycoplasma pneumoniae IgG, IgA and IgM EIA methods based on the enrichment of P1-protein (ThermoLabsystems, Helsinki, Finland) ( L) for the detection of acute infection. This evaluation was performed in two independent routine clinical microbiology laboratories. The first laboratory used samples preselected by IgG and IgM Platelia® enzyme immunoassay ( P) and the second used samples preseleced by Serion ELISA Classic M. pneumoniae IgG, IgM ( V). The L method was also compared to the FDA approved method of ImmunoWell® M. pneumoniae IgG and IgM ( G). When the agreement of two methods was applied as a serologic criteria for an acute infection, the following ratios of acute to nonacute infection were calculated 32/86 (totally 118) in the first and 20/72 (totally 92) in the second laboratory. In the first laboratory, the corresponding ratios by methods were 35/83 (sensitivity 100%, specificity 96.5%), 31/87 (sensitivity 97%, specificity 100%), and 55/63 (sensitivity 100%, specificity 79%) for the L, P and G methods, respectively. In the second laboratory, the ratios were 21/71 (sensitivity 100%, specificity 99%), 16/76 (sensitivity 83%, specificity 100%), and 53/39 (sensitivity 100, specificity 69%) for the L, V and G methods, respectively. Taking into account that the tested sample material was preselected by the P and V methods, which may have introduced some bias in their favor, the newly developed L method utilizing P1-enriched protein was found reliable for serodiagnosis of acute M. pneumoniae infection. The method G was the least specific in detection of acute infection.