Acute pancreatitis in aging animals： Loss of pancreatitis- associated protein protection

• Published in: World journal of gastroenterology : WJG Vol. 18; no. 26; pp. 3379 - 3388
• Main Authors: Fu, Sophia, Stanek, Albert, Mueller, Cathy M, Brown, Nefertti A, Huan, Chongmin, Bluth, Martin H, Zenilman, Michael E
• Format: Journal Article
• Language: English
• Published: United States Baishideng Publishing Group Co., Limited 14.07.2012
• Subjects: Acute Disease; Aging; Animals; Antigens, Neoplasm - biosynthesis; Biomarkers, Tumor - biosynthesis; C-Reactive Protein - metabolism; Genomics; Immunohistochemistry - methods; Inflammation; Lectins, C-Type - biosynthesis; Male; Original; Pancreatitis - diagnosis; Pancreatitis - metabolism; Pancreatitis-Associated Proteins; PAPS; Rats; Rats, Sprague-Dawley; Ribosomes - metabolism; RNA, Ribosomal - metabolism; Taurocholic Acid - pharmacology; Time Factors; 保护; 动物; 单核细胞趋化蛋白1; 急性胰腺炎; 相关蛋白; 聚合酶链反应; 血清淀粉酶; Aging; Rats; Pancreatitis-associated protein; Acute pancreatitis; Molecular biology
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v18.i26.3379

AIM：To investigate the effect of age on severity of acute pancreatitis（AP） using biochemical markers,histology and expression of the protective pancreatitisassociated proteins（PAPs）.METHODS：AP was induced via intraductal injection of 4% sodium taurocholate in young and old rats.Sera and pancreata were assayed at 24 h for the parameters listed above;we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal RNA.RESULTS：At 24 h after induction of AP,the pancreata of older animals had less edema（mean ± SE histologic score of young vs old：3.11 ± 0.16 vs 2.50 ±-0.11,P 〈 0.05）,decreased local inflammatory response（histologic score of stromal infiltrate：3.11 ± 0.27 vs 2.00 ± 0.17,P 〈 0.05） and increased bacterial infiltration（174% ± 52% increase from sham vs 377% ± 4%,P 〈 0.05）.A decreased expression of PAP1 and PAP2 was demonstrated by Western blotting analysis and immunohistochemical staining.There were no differences in serum amylase and lipase activity,or tissue myeloperoxidase or monocyte chemotactic protein-1 levels.However,in the most-aged group,serum C-reactive protein levels were higher（young vs old：0.249 ± 0.04 mg/dL vs 2.45 ± 0.68 mg/dL,P 〈 0.05）.CONCLUSION：In older animals,there is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level;this may explain the more aggressive clinical course.