A saturated genetic linkage map of rubber tree (Hevea spp.) based on RFLP, AFLP, microsatellite, and isozyme markers

• Published in: Theoretical and applied genetics Vol. 100; no. 1; pp. 127 - 138
• Main Authors: LESPINASSE, D, RODIER-GOUD, M, GRIVET, L, LECONTE, A, LEGNATE, H, SEGUIN, M
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.01.2000; Berlin Springer Nature B.V
• Subjects: Amplified fragment length polymorphism; Biological and medical sciences; Chromosome mapping; Classical genetics, quantitative genetics, hybrids; Cloning; Enzymes; Families & family life; Fundamental and applied biological sciences. Psychology; Genetic aspects; Genetic markers; Genetic testing; Genetics of eukaryotes. Biological and molecular evolution; Genomes; Hevea brasiliensis; Hybridization; Identification and classification; Linkage (Genetics); Plant genetics; Plant reproduction; Pteridophyta, spermatophyta; Rubber; Rubber trees; Seeds; segregation distortion; Vegetals; Yeast; France; Ivory Coast; Genetic mapping; Hevea brasiliensis; Laticiferous plant; Linkage; Genetic marker; Enzyme; Isozyme; Hybrid; Restriction fragment length polymorphism; Dicotyledones; Microsatellite DNA; Angiospermae; Interspecific hybridization; Euphorbiaceae; Spermatophyta; Hardwood forest tree
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220050018

The first genetic map for Hevea spp. (2n = 36) is presented here. It is based on a [F.sub.1] progeny of 106 individuals allowing the construction of a female, a male, and a synthetic map according to the pseudo-testcross strategy. Progeny were derived from an interspecific cross between PB260, a H. brasiliensis cultivated clone, and RO38, a H. brasiliensisxH. benthamiana interspecific hybrid clone. The disomic inheritance observed for all the codominant markers scattered on the 2n = 36 chromosomes revealed that Hevea behaves as diploids. Homologous linkage groups between the two parental maps were merged using bridge loci. A total of 717 loci constituted the synthetic map, including 301 RFLPs, 388 AFLPs, 18 microsatellites, and 10 isozymes. The markers were assembled into 18 linkage groups, thus reflecting the basic chromosome number, and covered a total distance of 2144 cM. Nine markers were found to be unlinked. Segregation distortion was rare (1.4%). Average marker density was 1 per 3 cM. Comparison of the distance between loci in the parental maps revealed significantly less meiotic recombination in the interspecific hybrid male parent than in the female parent. Hevea origin and genome organisation are discussed.