miR-210 regulates the interaction between pancreatic cancer cells and stellate cells
•Pancreatic stellate cells (PSCs) play a pivotal role in the progression of pancreatic cancer.•miR-210 was up-regulated in pancreatic cancer cells by co-culture with PSCs.•PSCs induced miR-210 expression through ERK- and PI3K/Akt-dependent pathways.•Inhibition of miR-210 expression decreased migrati...
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Published in | Biochemical and biophysical research communications Vol. 437; no. 3; pp. 433 - 439 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
02.08.2013
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Subjects | |
Online Access | Get full text |
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Summary: | •Pancreatic stellate cells (PSCs) play a pivotal role in the progression of pancreatic cancer.•miR-210 was up-regulated in pancreatic cancer cells by co-culture with PSCs.•PSCs induced miR-210 expression through ERK- and PI3K/Akt-dependent pathways.•Inhibition of miR-210 expression decreased migration and epithelial-mesenchymal transition.
There is accumulating evidence that pancreatic stellate cells (PSCs) promote the progression of pancreatic cancer. microRNAs (miRNAs) are small non-coding RNAs acting as negative regulators of gene expression at the post-transcriptional level. This study aimed to clarify the role of miRNAs in the interaction between PSCs and pancreatic cancer cells. Pancreatic cancer cells were mono-cultured or indirectly co-cultured with PSCs. miRNAs were prepared, and Agilent’s miRNA microarray containing probes for 904 human miRNAs was used to identify differentially expressed miRNAs. miR-210 was identified as an upregulated miRNA by co-culture with PSCs. Conditioned media of PSCs activated ERK and Akt, but not hypoxia-inducible factor-1α pathway. PSCs-induced miR-210 upregulation was inhibited by inhibitors of ERK and PI3K/Akt pathways. Inhibition of miR-210 expression decreased migration, decreased the expression of vimentin and snai-1, and increased the membrane-associated expression of β-catenin in Panc-1 cells co-cultured with PSCs. In conclusion, our results suggest a novel role of miR-210 in the interaction between PSCs and pancreatic cancer cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/j.bbrc.2013.06.097 |