Study of inflammatory factors’ effect on the endothelial barrier using piezoelectric biosensor

• Published in: Biosensors & bioelectronics Vol. 109; pp. 43 - 49
• Main Authors: Han, Junliang, Tong, Feifei, Chen, Ping, Zeng, Xiancai, Duan, Zhengyong
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 30.06.2018
• Subjects: Biosensing Techniques; Cells, Cultured; Electric Impedance; Endothelial Cells - chemistry; Endothelial Cells - pathology; Histamine - toxicity; Humans; Inflammation - chemically induced; Inflammation - diagnosis; Inflammation - pathology; Lipopolysaccharides - toxicity; Surface Properties
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2018.02.064

This paper used piezoelectric sensor to study the dysfunction of endothelial cell monolayer barrier caused by inflammatory factors. The biocompatible conductive polymer membrane of pPy[pGlu]-pLys was prepared on the surface of the ITO work electrode to improve the interface between the endothelial cell and the electrode. Both the impedance analysis data and the stable plateau stage of sensor's frequency shift indicated that endothelial cells formed a good monolayer barrier on this polymer surface. The response frequency shifts of lipopolysaccharide (LPS)- and histamine-induced endothelial barrier dysfunction were different, which distinguished their different stimulation mechanism. It provided a valuable analysis method for detecting the endothelial barrier function affected by inflammatory factor, and could further promote the application of piezoelectric sensor in cell biology and toxicology research. •The conductive polymer was adopted as the biomimetic substrate of the piezoelectric sensor.•The sensor was used to detect inflammatory factor's effect on the endothelial barrier.•It provided a valuable method and promote the application of piezoelectric sensor.