Resveratrol protects cardiomyocytes from oxidative stress through SIRT1 and mitochondrial biogenesis signaling pathways

• Published in: Biochemical and biophysical research communications Vol. 438; no. 2; pp. 270 - 276
• Main Authors: Li, Yong-guang, Zhu, Wei, Tao, Jian-ping, Xin, Ping, Liu, Ming-ya, Li, Jing-bo, Wei, Meng
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23.08.2013
• Subjects: Animals; Antioxidants - pharmacology; Cardiomyocytes; Cell Line; Cell Survival; Hydrogen Peroxide - pharmacology; Mitochondria; Mitochondria - metabolism; Mitochondrial Turnover; Myocytes, Cardiac - drug effects; NADH Dehydrogenase - physiology; Oxidative Stress; Rats; Reactive oxygen species; Reactive Oxygen Species - metabolism; Resveratrol; Signal Transduction; SIRT1; Sirtuin 1 - metabolism; Stilbenes - pharmacology; DMEM; Reactive oxygen species; Mitochondria; DMSO; FBS; DCF; ROS; Resveratrol; Cardiomyocytes; NAM; MTT; SIRT1; Dulbecco’s Modified Eagle Medium; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; nicotinamide; fluorescent dichlorofluorescein; fetal bovine serum; dimethyl sulfoxide
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2013.07.042

•Resveratrol induces mitochondrial biogenesis.•Resveratrol inhibits cardiomyocytes ROS production.•Resveratrol protects cardiomyocytes through SIRT1. Reactive oxygen species (ROS) is generated by oxidative stress and plays an important role in various cardiac pathologies. The SIRT1 signaling pathway and mitochondrial biogenesis play essential roles in mediating the production of ROS. SIRT1 activated by resveratrol protects cardiomyocytes from oxidative stress, but the exact mechanisms by which SIRT1 prevents oxidative stress, and its relationship with mitochondrial biogenesis, remain unclear. In this study, it was observed that after stimulation with 50μMH2O2 for 6h, H9C2 cells produced excessive ROS and downregulated SIRT1. The mitochondrial protein NDUFA13 was also downregulated by ROS mediated by SIRT1. Resveratrol induced the expression of SIRT1 and mitochondrial genes NDUFA1, NDUFA2, NDUFA13 and Mn-SOD. However, the production of these genes was reversed by SIRT1 inhibitor nicotinamide. These results suggest that resveratrol inhibits ROS generation in cardiomyocytes via SIRT1 and mitochondrial biogenesis signaling pathways.