Buckling of microtubules on elastic media via breakable bonds

• Published in: Biochemical and biophysical research communications Vol. 480; no. 1; pp. 132 - 138
• Main Authors: Afrin, Tanjina, Kabir, Arif Md. Rashedul, Sada, Kazuki, Kakugo, Akira, Nitta, Takahiro
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 04.11.2016
• Subjects: Buckling; Compressive Strength; Computer Simulation; Elastic foundation model; Elasticity; Kinesin; Kinesin - chemistry; Kinesin - metabolism; Mechanical stress; Microtubule; Microtubules - chemistry; Microtubules - metabolism; Elastic foundation model; Microtubule; Mechanical stress; Buckling; Kinesin
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2016.09.133

Buckling of microtubules observed in cells has been reconstructed on a two-dimensional elastic medium consisting of kinesins grafted over compressible substrates, enabling precise control of experimental conditions and quantitative analysis. However, interpretations of the observations have ambiguities due to inevitable experimental difficulties. In this study, with computer simulations, we investigated importance of the mode of interaction of microtubule with elastic medium in the buckling behavior of microtubule. By taking into consideration of forced-induced detachments of kinesins from microtubules, our simulations reproduced the previous experimental results, and showed deviations from predictions of the elastic foundation model. On the other hand, with hypothetical linkers permanently bound to microtubules, our simulation reproduced the predictions of the elastic foundation model. By analyzing the results of the simulations, we investigated as to why the difference arose. These findings indicate the importance of the mode of interaction of microtubule with the medium in the buckling behavior of microtubule. Our findings would bring new insights on buckling of microtubules in living cells. •Mode of interaction between microtubule (MT) and medium is significant in buckling.•Simulation deviated from the elastic foundation model in buckling with kinesin.•Our simulation reproduced the earlier experimental results.•Permanent interaction of MT to media followed the elastic foundation model.