Staining of pig lymphocytes subpopulations with acid α-naphthyl acetate esterase

• Published in: Veterinary immunology and immunopathology Vol. 14; no. 2; pp. 173 - 179
• Main Authors: Salmon, H., Licence, S.T., Binns, R.M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.1987; Elsevier
• Subjects: Animals; B-Lymphocytes - classification; B-Lymphocytes - enzymology; B-Lymphocytes - immunology; Life Sciences; Lymphocytes - classification; Lymphocytes - enzymology; Lymphocytes - immunology; Naphthol AS D Esterase - blood; Receptors, Complement; Receptors, Fc; Receptors, IgG; Rosette Formation; Staining and Labeling; Swine - blood; T-Lymphocytes - classification; T-Lymphocytes - enzymology; T-Lymphocytes - immunology
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/0165-2427(87)90051-1

The use of α-naphthyl acetate esterase (ANAE) as a T cell marker in some other species and the broad correlation of incidence of ANAE-positivity and E rosette-formation in the pig suggest that ANAE-staining may be a T-cell marker in the pig. However, by studying the staining of lymphocytes within a variety of rosettes in fixed preparations a similar incidence of pig blood lymphocytes were found to be ANAE + among T cells (E rosettes formed in dextran), B cells antiglobulin rosettes) and Fc-γ receptor-bearing B and T cells (EA rosettes in saline and dextran): complement (C′) receptor-bearing cells showed a higher incidence of staining than other lymphocytes. Analysis of staining morphology suggested that certain morphologies within the B and T lineages may be confined to subpopulations. Thus ANAE positivity is certainly not a marker identifying blood T lymphocytes but could be of some value indicating subpopulations of B and T lymphocytes.