Effect of silver nanoparticles and REP-PCR typing of Staphylococcus aureus isolated from various sources

• Published in: Scientific reports Vol. 14; no. 1; pp. 21997 - 13
• Main Authors: Elghazaly, Eman M., Torky, Helmy A., Tawfik, Rasha Gomaa
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23.09.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/326; 631/337; Ag-NPs; Animals; Antibiotic resistance; Biofilm; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Cell surface; COVID-19; COVID-19 - microbiology; COVID-19 - veterinary; COVID-19 - virology; Electron microscopy; Food; Food contamination; Food contamination & poisoning; Food Microbiology; Goats; Humanities and Social Sciences; Humans; Metal Nanoparticles; Microbial Sensitivity Tests; Morel's diseased; multidisciplinary; Multidrug resistance; Nanoparticles; Polymerase Chain Reaction - methods; REP-PCR; SARS-CoV-2 - genetics; SARS-CoV-2 - isolation & purification; Science; Science (multidisciplinary); Sheep; Silver; Silver - pharmacology; Staphylococcal Infections - microbiology; Staphylococcal Infections - veterinary; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification; Strains (organisms); Zoonoses; COVID-19; Biofilm; Electron microscopy; REP-PCR; Ag-NPs; Morel's diseased
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-71781-w

This is the primary study at Matrouh Governorate to unveil antibiotic resistance, biofilm formation, silver nanoparticles (Ag-NPs) effect using electron microscopy, and REP-PCR analysis of Staphylococcus aureus strains isolated from COVID-19 patients, contaminated food, and Morel’s diseased sheep and goats. A total of 15 S. aureus strains were isolated; five from each of the COVID-19 patients, Morel's diseased sheep and goats, and contaminated food. All strains were considered multidrug-resistant (MDR). All strains showed the presence of biofilm. Morphological changes in the cell surface of the bacterium were evidenced, and penetration with the rupture of some bacterial cells. Based on REP-PCR analysis, 4 clusters (C1-C4) with dissimilarity between clusters C1 and C2 8% and between C3 and C4 15%. Cluster I included 3 strains from contaminated food with a similarity of 97%, and Cluster II included 2 strains from contaminated food and 2 from COVID-19-infected patients with a similarity of 96% (confirming the zoonotic nature of this pathogen). Cluster III contained 4 strains isolated from Morel's diseased sheep & goats with a similarity ratio of 99% in comparison the 4th cluster contained 3 strains isolated from COVID-patients and one from Morel's diseased sheep & goats with a similarity ratio of 92%.