Mutations of the gyrA gene of clinical isolates of Salmonella typhimurium and three other Salmonella species leading to decreased susceptibilities to 4-quinolone drugs

• Published in: Journal of antimicrobial chemotherapy Vol. 37; no. 2; pp. 351 - 356
• Main Authors: Brown, J. C., Thomson, C. J., Amyes, S. G. B.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.02.1996
• Subjects: Amino Acid Sequence; Anti-Infective Agents - pharmacology; Bacteriology; Base Sequence; Biological and medical sciences; Ciprofloxacin - pharmacology; Drug Resistance, Microbial; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetics; India; Microbial Sensitivity Tests; Microbiology; Molecular Sequence Data; Point Mutation; Salmonella - drug effects; Salmonella - genetics; Salmonella - isolation & purification; Salmonella typhimurium; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics; Salmonella typhimurium - isolation & purification; United Kingdom; Quinolone derivative; Salmonella; Enzyme; DNA topoisomerase; Salmonella typhimurium; Resistance; Antibiotic; Isomerases; Bacteria; Mutation; Antibacterial agent; Clinical isolate; Aminoacid sequence; Enterobacteriaceae
• ISSN: 0305-7453; 1460-2091
• DOI: 10.1093/jac/37.2.351

The region of the gyrA gene encoding nucleotides 72 to 557 in ten Salmonella tvphimurium clinical isolates received from Vellore, India and six British clinical isolates of a number of Salmonella species has been sequenced. Those exhibiting a decreased susceptibility to 4-quinolone drugs (MICs of ciprofloxacin ranging from 0.128 mg/L to 1.0 mg/L) have been shown to possess a number of different mutations in this region of the gene (Asp-87 to Gly, Asp-87 to Asn and Ser-83 to Phe). The mutations Asp-87 to Gly and Ser-83 to Phe/Asp-87 to Asn have yet to be reported occurring in S. typhimurium