Gene expression profiling of ozone-treated Arabidopsis abi1td insertional mutant: protein phosphatase 2C ABI1 modulates biosynthesis ratio of ABA and ethylene

• Published in: Planta Vol. 230; no. 5; pp. 1003 - 1017
• Main Authors: Ludwików, Agnieszka, Kierzek, Dorota, Gallois, Patrick, Zeef, Leo, Sadowski, Jan
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.10.2009; Springer; Springer Nature B.V
• Subjects: Abscisic Acid - biosynthesis; Agriculture; Alternative Splicing - drug effects; Alternative Splicing - genetics; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Biological and medical sciences; Biological Transport - drug effects; Biomedical and Life Sciences; Biosynthesis; Cyclopentanes - pharmacology; Drought; Droughts; Ecology; Ethylenes - biosynthesis; Forestry; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; Gene expression regulation; Gene Expression Regulation, Plant - drug effects; Genes; Hypersensitivity; Life Sciences; Mutagenesis, Insertional - drug effects; Mutation - genetics; Original Article; Oxylipins - pharmacology; Ozone; Ozone - pharmacology; Phenotype; Phosphatases; Phosphoprotein Phosphatases - genetics; Phosphoprotein Phosphatases - metabolism; Plant cells; Plant Sciences; Plants; Protein Phosphatase 2C; Proteins; Regulon - genetics; Reproducibility of Results; Signal Transduction - drug effects; Signal Transduction - genetics; Stress response; Stress, Physiological - drug effects; Transcription, Genetic - drug effects; DNA microarray; ABI1 protein phosphatase 2C; ABA and ethylene biosynthesis; Drought and ozone; Hormone cross-talk; Phosphoprotein phosphatase; Ethylene; DNA chip; Phosphoric monoester hydrolases; Plant biology; Esterases; Biosynthesis; Arabidopsis thaliana; Cruciferae; Dicotyledones; Sesquiterpenes; Modulation; Angiospermae; Abscisic acid; Plant growth substance; Ratio; Drought; Hormone; Enzyme; Ozone; Gene expression; Hydrolases; Spermatophyta; Mutation; Experimental plant
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-009-1001-8

We report on the characterization of the interaction between reactive oxygen species signalling and abscisic acid (ABA)-mediated gene network in ozone (O3) stress response. To identify the stress-related signalling pathways and possible cross-talk controlled by an ABA-negative regulator, the protein phosphatase 2C abscisic acid insensitive1 (ABI1), we performed a genome-wide transcription profiling of O3-treated wild-type and ABI1 knockout (abi1td) plants. In addition, to better understand ABA signalling and the interactions between stress response pathways, we performed a microarray analysis of drought-treated plants. Functional categorization of the identified genes showed that ABI1 is involved in the modulation of several cellular processes including metabolism, transport, development, information pathways and variant splicing. Comparisons with available transcriptome data sets revealed the extent of ABI1 involvement in both ABA-dependent and ABA-independent gene expression. Furthermore, in O3 stress the ABA hypersensitivity of abi1td resulted in a significant reduction of the ABA level, ethylene (ET) over-production and O3 tolerance. Moreover, the physical interaction of ABI1 with ACC synthase2 and ACC synthase6 was shown. We provide a model explaining how ABI1 can regulate both ABA and ET biosynthesis. Altogether, our findings indicate that ABI1 plays the role of a general signal transducer linking ABA and ET biosynthesis as well as signalling pathways to O3 stress tolerance.