siRNA targeting of Cdx2 inhibits growth of human gastric cancer MGC-803 cells

• Published in: World journal of gastroenterology : WJG Vol. 18; no. 16; pp. 1903 - 1914
• Main Authors: Wang, Xiao-Tong, Xie, Yu-Bo, Xiao, Qiang
• Format: Journal Article
• Language: English
• Published: United States Baishideng Publishing Group Co., Limited 28.04.2012
• Subjects: Animals; Caspase 3 - metabolism; Caspase 9 - metabolism; caspase-3; CDX2 Transcription Factor; Cell Cycle; Cell Line, Tumor; Cell Movement; Cell Proliferation; Homeodomain Proteins - antagonists & inhibitors; Homeodomain Proteins - physiology; Humans; Male; Mice; Mice, Inbred BALB C; Neoplasm Invasiveness; Original; PTEN Phosphohydrolase - analysis; PTEN基因; RNA, Messenger - analysis; RNA, Small Interfering - genetics; RT-PCR法; siRNA; Stomach Neoplasms - pathology; Stomach Neoplasms - therapy; Transcription Factors - antagonists & inhibitors; Transcription Factors - physiology; 反转录聚合酶链反应; 细胞周期; 细胞生长; 胃癌细胞; Cdx2; Small interference RNA; Growth; Gastric cancer
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v18.i16.1903

AIM：To investigate the effects of small interference RNA（siRNA） targeting of Cdx2 on human gastric cancer MGC-803 cells in vitro and in vivo.METHODS：The recombinant pSilencer 4.1-Cdx2 siRNA plasmids were constructed and transfected into gastric cancer MGC-803 cells in vitro.The stable transfectants were selected.The effects of Cdx2 siRNA on growth,proliferation,cell cycle,apoptosis,migration and invasiveness of human gastric cancer MGC-803 cells were evaluated and the expression of phosphatase and tensin homolog（PTEN）,caspase-9 and caspase-3 was observed in vitro by reverse transcription polymerase chain reaction（RT-PCR） and Western blotting analysis.We also investigated the effect of Cdx2 siRNA on growth of MGC-803 cells in nude mice in vivo.RESULTS：Cdx2 siRNA led to inhibition of endogenous Cdx2 mRNA and protein expression as determined by RT-PCR and Western blotting analysis.Cdx2 siRNA significantly inhibited cell growth and proliferation,blocked entry into the S-phase of the cell cycle,induced cell apoptosis,and reduced the motility and invasion of MGC-803 cells.Cdx2 siRNA also increased PTEN expression,and activated caspase-9 and caspase-3 in MGC-803 cells in vitro.In addition,siRNA targeting of Cdx2 inhibited the growth of MGC-803 cells and promoted tumor cell apoptosis in vivo in nude mice tumor models.CONCLUSION：Cdx2 was involved in regulating progression of human gastric cancer cells MGC-803.Manipulation of Cdx2 expression may be a potential therapeutic strategy for gastric cancer.