The p48 subunit of the damaged-DNA binding protein DDB associates with the CBP/p300 family of histone acetyltransferase

• Published in: Mutation research Vol. 486; no. 2; pp. 89 - 97
• Main Authors: Datta, Abhishek, Bagchi, Srilata, Nag, Alo, Shiyanov, Pavel, Adami, Guy R, Yoon, Taewon, Raychaudhuri, Pradip
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 12.07.2001; Elsevier
• Subjects: Acetyltransferases - metabolism; Biological and medical sciences; CBP protein; CBP/p300; DDB; DDB protein; DNA Damage; DNA Repair; DNA-Binding Proteins - metabolism; E2F protein; Fundamental and applied biological sciences. Psychology; Histone Acetyltransferases; Humans; Models, Genetic; Molecular and cellular biology; Molecular genetics; Mutagenesis. Repair; Nuclear Proteins - metabolism; p300 protein; p48 protein; Protein Binding; Saccharomyces cerevisiae Proteins; Trans-Activators - metabolism; Tumor Cells, Cultured; DNA damage; CBP/p300; DDB; Human; Acyltransferases; Chromatin; Enzyme; Transferases; Molecular interaction; Subunit; Damaged-DNA binding protein; Histone acetyltransferase; Cell line; DNA; Cell cycle; Lesion; Repair
• ISSN: 0921-8777; 0027-5107; 1386-1476
• DOI: 10.1016/S0921-8777(01)00082-9

DDB has been implicated in DNA repair as well as transcription. Mutations in DDB have been correlated with the repair-deficiency disease, xeroderma pigmentosum group E (XP-E). The XP-E cells exhibit deficiencies in global genomic repair, suggesting a role for DDB in that process. DDB also possesses a transcription stimulatory activity. We showed that DDB could function as a transcriptional partner of E2F1. But the mechanism by which DDB stimulates E2F-regulated transcription or carry out its DNA repair function is not understood. To investigate the mechanisms, we looked for nuclear proteins that interact with DDB. Here we show that DDB associates with the CBP/p300 family of proteins, in vivo and in vitro. We suggest that DDB participates in global genomic repair by recruiting CBP/p300 to the damaged-chromatin. It is possible that the histone acetyltransferase activities of the CBP/p300 proteins induce chromatin remodeling at the damaged-sites to allow recruitment of the repair complexes. The observation offers insights into both transcription and repair functions of DDB.