Restoration of stress-induced altered T cell function and corresponding cytokines patterns by Withanolide A

• Published in: International immunopharmacology Vol. 9; no. 10; pp. 1137 - 1144
• Main Authors: Kour, Kiranjeet, Pandey, Anjali, Suri, K.A., Satti, N.K., Gupta, K.K., Bani, Sarang
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.09.2009; Elsevier
• Subjects: Administration, Oral; Alanine Transaminase - biosynthesis; Alanine Transaminase - blood; Alanine Transaminase - genetics; Animals; Aspartate Aminotransferases - biosynthesis; Aspartate Aminotransferases - blood; Aspartate Aminotransferases - genetics; Biological and medical sciences; Chronic stress; Corticosterone - genetics; Corticosterone - metabolism; Ergosterol - administration & dosage; Ergosterol - analogs & derivatives; Flow cytometry; Hepatocytes - drug effects; Hepatocytes - immunology; Hepatocytes - metabolism; Hepatocytes - pathology; Immunophenotyping; Interferon-gamma - secretion; Interleukin-2 - secretion; Intracellular cytokines; Lipid Peroxidation - drug effects; Medical sciences; Mice; Pharmacology. Drug treatments; Restraint, Physical; Stress, Physiological - drug effects; Stress, Physiological - immunology; T-Lymphocyte Subsets - drug effects; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - metabolism; T-Lymphocyte Subsets - pathology; Withania; Withanolides; Intracellular cytokines; Flow cytometry; Immunophenotyping; Chronic stress; lmmunophenotyping; Chronic; Cell function; Cytokine; T-Lymphocyte; Intracellular; Stress
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2009.05.011

This study was taken up to see the effect of Withanolide A (WS-1), a compound isolated from Withania somnifera root extract on chronic stress-induced alterations on T lymphocyte subset distribution and corresponding cytokine secretion patterns in experimental Swiss albino mice. Stress disturbs the homeostatic state of the organism and brings about behavioral, endocrine and immunological changes. The chronic suppression induced by stress depresses the immune functioning and increases susceptibility to diseases. Oral administration of WS-1 once daily at the graded doses of 0.25, 0.5, 1 and 2 mg/kg p.o. caused significant recovery of stress-induced depleted T cell population causing an increase in the expression of IL-2 and IFN-gamma (a signature cytokine of Th1 helper cells) and a decrease in the concentration of corticosterone in stressed experimental animals. It also reversed the restraint stress-induced increase in plasma alanine aminotransferase (ALT), aspartate aminotransferase(AST) and hepatic lipid peroxidation (LP) levels and improved the restraint stress-induced decrease in hepatic glutathione (GSH), and glycogen levels, thus showing the significant antistress potential of the test drug.