Activity in MCF-7 Estrogen-sensitive Breast Cancer Cells of Capsicodendrin from Cinnamosma fragrans

• Published in: Anticancer research Vol. 41; no. 12; pp. 5935 - 5944
• Main Authors: Acuna, Ulyana Munoz, Ezzone, Nathan, Rakotondraibe, L Harinantenaina, Carcache DE Blanco, Esperanza J
• Format: Journal Article
• Language: English
• Published: Greece International Institute of Anticancer Research 01.12.2021
• Subjects: Anticancer properties; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - pharmacology; Antitumor agents; Biochemistry; Biokemi; Breast cancer; Capsicodendrin; Caspase-1; Caspase-7; Caspases - metabolism; Cell cycle; Cell Cycle - drug effects; Cell death; Cinnamomum - chemistry; Cytotoxicity; Electric potential; Estrogens; Farmakologi; Female; Fluorescence; Fluorescent indicators; Humans; I-kappa B Kinase; ICAM-1; IKK beta; Intercellular adhesion molecule 1; MCF-7; MCF-7 Cells - drug effects; Membrane potential; Membrane Potential, Mitochondrial - drug effects; Metastases; Mitochondria; Molecular Structure; NF-kappa B; NF-kappa B - metabolism; NF-κB protein; Pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Reactive oxygen species; Reactive Oxygen Species - metabolism; ROS; Signal Transduction - drug effects; NF-κB; caspase-7; MCF-7; ROS; Capsicodendrin; ICAM-1; IKKβ; caspase-1
• ISSN: 0250-7005; 1791-7530; 1791-7530
• DOI: 10.21873/anticanres.15412

Effect of capsicodendrin on the NF-κB pathway was studied in MCF-7 cancer cells. The transcription factor assay was used to screen for NF-κB activity. The effect on IKKβ, ICAM-1, and caspase-7 were studied using western blot. Caspase-1 was studied using Promega Caspase-Glo assay. Reactive oxygen species (ROS) were detected using the fluorescent probe DCFH-DA. The potentiometric dye JC-1 was used to assess mitochondrial membrane potential (ΔΨm) and the cell cycle was examined using a fluorescence-activated cell sorter. NF-κB p65 inhibitory effect was IC =8.6 μM and cytotoxic activity was IC =7.5 μM. The upstream IKK and the downstream ICAM-1 were down-regulated. Sub G -phase population increased to 81% after 12 h of treatment with capsicodendrin (10 μM) and there was no loss of ΔΨM. Increased levels of intracellular ROS promoted activity of caspase-1 and induced cell death in MCF-7 cells. Capsicodendrin may be a future anticancer agent that prevents the progression of metastatic breast cancer.