Recombinant Microdochium nivale carbohydrate oxidase and its application in an amperometric glucose sensor

• Published in: Biosensors & bioelectronics Vol. 16; no. 4; pp. 319 - 324
• Main Authors: Kulys, Juozas, Tetianec, Lidija, Schneider, Palle
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.06.2001; Elsevier Science
• Subjects: Aspergillus niger; Biological and medical sciences; Biosensing Techniques; Biosensor; Biosensors; Biotechnology; Carbohydrate; Carbohydrate Dehydrogenases - chemistry; Carbohydrate Dehydrogenases - genetics; Fundamental and applied biological sciences. Psychology; Glucose; Glucose - analysis; glucose oxidase; Mediator; Methods. Procedures. Technologies; Microdochium nivale; Oxidase; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Various methods and equipments; Aspergillus niger; Biosensor; Oxidase; Carbohydrate; Mediator; Glucose; Microdochium nivale; Performance evaluation; Glucose oxidase; Enzyme; Carbon electrode; Enzyme electrode; Fungi; Chemical modification; Graphite; Amperometry; Paste electrode; Fungi Imperfecti; Oxidoreductases; Recombinant protein; Comparative study; Thallophyta; Quantitative analysis
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/S0956-5663(01)00128-2

Biosensors containing recombinant carbohydrate oxidase from Microdochium nivale (rMnO) were developed by means of either chemically modified carbon paste or graphite electrode. 1-( N, N-dimethylamine)-4-(4-morpholine)benzene (AMB) and 1,1′-dimethylferrocene (DMFc) have been used as mediators. The biosensors showed a linear calibration graph up to 18 mM of glucose when operated at 0.04–0.36 V versus a saturated calomel electrode. Almost no change was detected in the sensitivity of the biosensors at pH 7.2–8.1. The biosensors responded to other aldoses in the d-configuration, however, maximal sensitivity of the biosensor was towards d-glucose. The biosensor did not response to polyhydroxylic compounds such as d-mannitol, d-sorbitol and inositol. The advantages of the biosensors based on rMnO in comparison to Aspergillus niger glucose oxidase is a wider linear range, low sensitivity to oxygen and (in some cases) broad specificity.