Study of the authenticity of commercial wine yeast strains by molecular techniques
mtDNA restriction analysis has been carried out with 45 different commercial Saccharomyces wine yeast strains. The analysis with Hinf I provided unique profiles for 17 of the 45 strains and can therefore be considered as individual strains. Nevertheless, among the remaining 28 strains, only eight mt...
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Published in | International journal of food microbiology Vol. 70; no. 1; pp. 1 - 10 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
22.10.2001
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | mtDNA restriction analysis has been carried out with 45 different commercial
Saccharomyces wine yeast strains. The analysis with
Hinf I provided unique profiles for 17 of the 45 strains and can therefore be considered as individual strains. Nevertheless, among the remaining 28 strains, only eight mtDNA restriction patterns appeared. These strains were subjected to electrophoretic karyotyping and PCR amplification of δ sequences. We concluded that the maximum discriminatory power was obtained when the results of the three techniques were combined, giving 13 different composite patterns for the 28 strains under study. The results showed evidence of mistakes during production or fraudulent practices by yeast producers, since only 30 individual strains have been identified among the 45
Saccharomyces wine yeast strains commercialised by different companies. Additionally, commercial starters of
Saccharomyces uvarum and
Saccharomyces bayanus have been re-identified as
Saccharomyces cerevisiae. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0168-1605 1879-3460 |
DOI: | 10.1016/S0168-1605(01)00502-5 |