QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups

• Published in: Theoretical and applied genetics Vol. 101; no. 1-2; pp. 173 - 184
• Main Authors: MARQUEZ-CEDILLO, L. A, HAYES, P. M, JONES, B. L, KLEINHOFS, A, LEGGE, W. G, ROSSNAGEL, B. G, SATO, K, ULLRICH, E, WESENBERG, D. M
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.07.2000; Berlin Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Analysis; Barley; Biological and medical sciences; chromosome 2; chromosome 4; Chromosomes; Enzymes; Fundamental and applied biological sciences. Psychology; Genetic aspects; Genetics and breeding of economic plants; Genomes; Germplasm; Haploidy, in vitro culture applications, somatic hybrids; Hordeum vulgare; Identification and classification; int-c gene; Plant breeding: fundamental aspects and methodology; Plant genetics; Proteins; Quantitative trait loci; Soil sciences; vrs1 gene; United States; Canada; United States > US; Genetic mapping; Barley; Monocotyledones; Hordeum vulgare; Quantitative character; Malting; Chromosome; Molecular interaction; Progeny; Germplasm; Phenotype; Gramineae; Quality; Angiospermae; Spermatophyta; Doubled haploid
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051466

Characterization of the determinants of economically important phenotypes showing complex inheritance should lead to the more effective use of genetic resources. This study was conducted to determine the number, genome location and effects of QTLs determining malting quality in the two North American barley quality standards. Using a doubled-haploid population of 140 lines from the cross of Harrington x Morex, malting quality phenotype data sets from eight environments, and a 107marker linkage map, QTL analyses were performed using simple interval mapping and simplified composite interval mapping procedures. Seventeen QTLs were associated with seven grain and malting quality traits (percentage of plump kernels, test weight, grain protein percentage, soluble/total protein ratio, α-amylase activity, diastatic power and malt-extract percentage). QTLs for multiple traits were coincident. The loci controlling inflorescence type [vrsl on chromosome 2(2H) and int-c on chromosome 4(4H)] were coincident with QTLs affecting all traits except malt-extract percentage. The largest effect QTLs, for the percentage of plump kernels, test weight protein percentage, S/T ratio and diastatic power, were coincident with the vrsl locus. QTL analyses were conducted separately for each sub-population (six-rowed and two-rowed). Eleven new QTLs were detected in the subpopulations. There were significant interactions between the vrsl and int-c loci for grain-protein percentage and S/T protein ratio. Results suggest that this mating of two different germplasm groups caused a disruption of the balance of traits. Information on the number, position and effects of QTLs determining components of malting quality may be useful for maintaining specific allele configurations that determine target quality profiles.