Human Jurkat lymphocytes clones differ in their capacity to support productive human immunodeficiency virus type 1 multiplication

• Published in: Journal of virological methods Vol. 92; no. 2; pp. 207 - 213
• Main Authors: Cervantes-Acosta, Guillermo, Cohen, Éric A., Lemay, Guy
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.04.2001; Amsterdam Elsevier; New York, NY
• Subjects: Animal viral diseases; Biological and medical sciences; Clone Cells - virology; Fundamental and applied biological sciences. Psychology; Giant Cells - physiology; HIV; HIV Infections - virology; HIV-1 - genetics; HIV-1 - pathogenicity; HIV-1 - physiology; Human immunodeficiency virus 1; Human Immunodeficiency Virus Proteins; Human lymphocytes; Human viral diseases; Humans; Infectious diseases; Jurkat cells; Jurkat Cells - virology; MAGI assay; Medical sciences; Microbiology; Transfection; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Viral infectivity; Viral Regulatory and Accessory Proteins - genetics; Viral Regulatory and Accessory Proteins - metabolism; Virus Replication - physiology; Vpu protein; Jurkat cells; Vpu protein; Viral infectivity; HIV; Human lymphocytes; MAGI assay; Human; Productivity; Immunopathology; Virus multiplication; HIV-1 virus; Host specificity; Retroviridae; AIDS; Lentivirus; Immune deficiency; Infection; Virus; Cell line; Viral disease; Infectivity; Replication; Human immunodeficiency virus; Cell; Lymphocyte; Stock; Clone
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/S0166-0934(00)00289-5

CD4-positive human lymphocytic cell lines are essential tools for the study of human immunodeficiency virus (HIV) replication. Jurkat cells are among cells more frequently used for this purpose. In the current study, various cell clones or cells stocks derived from this cell line were shown to vary substantially in their response to viral infection and in their ability to support productive virus multiplication. The formation of syncytia, the effect of Vpu on viral export, and especially the specific infectivity of the viruses released, can vary significantly among independent cell stocks. This suggests that the choice of an adequate cell clone or cell line could be critical while evaluating specific properties of the virus and further stresses the limitations of tissue culture models. However, these observations also raise the possibility of exploiting these differences among cells to study specific aspects of host-cell interactions contributing to viral multiplication.