Two-generation reproductive study in mink fed diisopropyl methylphosphonate (DIMP)

• Published in: Reproductive toxicology (Elmsford, N.Y.) Vol. 17; no. 3; pp. 327 - 334
• Main Authors: Bucci, Thomas J., Kovatch, Robert M., Mercieca, Michael D., Perman, Victor, Klingensmith, J.S.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.05.2003; Elsevier
• Subjects: Administration, Oral; Animals; Biological and medical sciences; Body Weight - drug effects; Chemical and industrial products toxicology. Toxic occupational diseases; Cholinesterases - blood; Diisopropyl methylphosphonate; DIMP; Dose-Response Relationship, Drug; Female; Heinz Bodies - metabolism; Male; Medical sciences; Mink; Mink - blood; Mink - growth & development; Mink - physiology; Mustela vison; No-Observed-Adverse-Effect Level; Oral toxicity; Organ Size - drug effects; Organophosphorus Compounds - toxicity; Pregnancy; Prenatal Exposure Delayed Effects; Reproduction - drug effects; Reproduction - physiology; Reproductive toxicity; Spleen - drug effects; Spleen - growth & development; Toxicology; Two-generation; Various organic compounds; Water Pollutants, Chemical - toxicity; Mustela vison; Reproductive toxicity; DIMP; Two-generation; Oral toxicity; Diisopropyl methylphosphonate; Mink; Fissipedia; Carnivora; Reproduction diseases; Toxicity; Oral administration; Multigeneration study; Vertebrata; Mammalia; Animal
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/S0890-6238(03)00004-2

Two generations of “Ranch Wild” mink ( Mustela vison) were fed the organophosphate diisopropyl methylphosphonate (DIMP) at 0, 150, 450, or 1250 ppm, to determine potential toxicity to the dams. Chemical, hematologic, necropsy, and microscopic examinations were performed on all parental animals and representative kits. The F0 and F1 dams had 3.4 and 4.6% mortality, respectively, distributed among all groups and not attributed to DIMP exposure. Adverse effects were mild and limited to the highest dose group. Plasma cholinesterase was reduced 40% (F0) and 31% (F1), as was whole blood cholinesterase (16 and 8.5%). Heinz bodies were present in 2.8% (F0) and 1.3% (F1) of erythrocytes. The erythrocyte count was reduced 6.3% in the F0. Reproductive efficiency was not affected. The mink were not uniquely susceptible to DIMP, relative to the literature on other species. The no observed adverse effect level (NOAEL), based on the 450 ppm group of F1 females, was 56.5 mg DIMP/kg BW per day; the lowest observed adverse effect level (LOAEL) was 329.5 mg DIMP/kg BW per day.