In vitro toxicity testing of supramolecular sensitizers for photodynamic therapy

• Published in: Toxicology in vitro Vol. 17; no. 5; pp. 775 - 778
• Main Authors: Kolárová, H, Mosinger, J, Lenobel, R, Kejlová, K, Jı́rová, D, Strnad, M
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2003; Elsevier Science
• Subjects: Biological and medical sciences; Cell Line, Tumor; Cell Survival - drug effects; Cell Survival - radiation effects; Dose-Response Relationship, Drug; Humans; Infrared Rays; Inhibitory Concentration 50; Light; Medical sciences; Melanoma - drug therapy; Melanoma - pathology; Melanoma - radiotherapy; meso-tetrakis(4-sulphonatophenyl)porphine; Microscopy, Fluorescence; Photochemotherapy - methods; Photodynamic therapy; Photoradiation therapy and photosensitizing agent; Phototoxicity; Porphyrins - toxicity; Radiation-Sensitizing Agents - toxicity; Sensitizers; Skin Neoplasms - drug therapy; Skin Neoplasms - pathology; Skin Neoplasms - radiotherapy; Toxicity Tests - methods; Treatment with physical agents; Treatment. General aspects; Tumors; zinc metallocomplex; TPPS4; PDT; CD; IC 50; Photodynamic therapy; Light; Phototoxicity; ZnTPPS4; Sensitizers; HP-β-CD; Human; Toxicity; Cytotoxicity; Transition metal Complexes; In vitro; Photochemotherapy; Non metal porphyrin; Cyclodextrin; Cell line; Malignant melanoma; Metalloporphyrin; Inclusion compound; Zinc Complexes; Photosensitizer; Tumor cell; Fluorescence microscopy
• ISSN: 0887-2333; 1879-3177
• DOI: 10.1016/S0887-2333(03)00094-8

We report the phototoxicity of meso-tetrakis(4-sulphonatophenyl)porphine (TPPS4) and zinc metallocomplex (ZnTPPS4) sensitizers in the presence or absence of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) on G361human melanoma cells. Morphological changes in cell cultures have been evaluated using inversion fluorescent microscope and image analysis. Viability of cells was determined by means of molecular probes for fluorescence microscopy (LIVE/DEAD kit- double staining with Calcein AM and Ethidium Homodimer). The quantitative changes of cell viability in relation to sensitizers concentrations and irradiation doses were proved by fluorometric measurement with fluoroscan Ascent. We found that the most effective sensitizer is ZnTPPS4 bound to HP-β-CD, since the IC 50 value was 12.5 g/ml at the dose of light radiation of 10 J/cm 2.