Antifungal activities of secondary metabolites isolated from liquid fermentations of Stereum hirsutum (Sh134-11) against Botrytis cinerea (grey mould agent)

• Published in: Food and chemical toxicology Vol. 109; no. Pt 2; pp. 1048 - 1054
• Main Authors: Aqueveque, Pedro, Céspedes, Carlos L., Becerra, José, Aranda, Mario, Sterner, Olov
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2017
• Subjects: Analytical Chemistry; Analytisk kemi; Antifungal; Basic Medicine; Basidiomycota - chemistry; Basidiomycota - metabolism; Botrytis; Botrytis - drug effects; Botrytis - growth & development; Chemical Sciences; Chilean fungi; Farmakologi och toxikologi; Fungicides, Industrial - chemistry; Fungicides, Industrial - metabolism; Fungicides, Industrial - pharmacology; Kemi; Medical and Health Sciences; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; Mycelium - drug effects; Mycelium - growth & development; Natural Sciences; Naturvetenskap; Pharmacology and Toxicology; Secondary Metabolism; Spores, Fungal - drug effects; Spores, Fungal - growth & development; Sterenin D; Stereum; Submerged cultures; Botrytis; Stereum; Antifungal; Submerged cultures; Chilean fungi; Sterenin D
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2017.05.036

Extracts obtained from liquid mycelial fermentations of the Chilean fungus Stereum hirsutum (Sh134-11) showed antifungal activity against Botrytis cinerea. Two types of extracts were obtained: EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extracts were more active than MeOH-extracts. A large-scale fermentation of Sh134-11 and chromatographic methods allowed to isolated four compounds: MS-3, Vibralactone, Vibralactone B and Sterenin D. Only Sterenin D showed antifungal activity against B. cinerea in the tests performed. Effects on the mycelial growth of B. cinerea showed that Sterenin D showed inhibition at 1000–2000 μg/mL reaching 67% and 76% respectively. Sterenin D was more effective to control the sporogenesis, inhibiting in 96% the sporulation at 500 μg/mL. Assays showed that Sterenin D exhibited a minimal fungicidal concentration (MFC) of 50 μg/mL and minimal inhibitory concentration (MIC) at 20 μg/mL. Our study indicated that submerged fermentations of Chilean S. hirsutum (Sh134-11) produced extracts with antifungal activity and Sterenin D is responsible for this activity, which could be used as possible biofungicides alternative to synthetic fungicides. [Display omitted] •Large-scale fermentation of Chilean fungus S. hirsutum (Sh-134-11) produced active extracts against B. cinerea.•Bioassay-guided fractionation allowed to identify 4 compounds: MS-3, Vibralactone, Vibralactone B and Sterenin D.•Only sterenin D showed antifungal activity inhibiting mycelial growth and sporogenesis of B. cinerea.•Sterenin D showed MIC and MFC values at 10 and 50 μg/mL against B. cinerea respectively.