Intravenous administration of replication‐incompetent adenovirus to rhesus monkeys induces thrombocytopenia by increasing in vivo platelet clearance

• Published in: British journal of haematology Vol. 123; no. 5; pp. 903 - 905
• Main Authors: Wolins, N., Lozier, J., Eggerman, T.L., Jones, E., Aguilar‐Córdova, E., Vostal, J. G.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.12.2003; Blackwell; Blackwell Publishing Ltd
• Subjects: Adenoviridae - genetics; Adenoviridae Infections - complications; adenovirus; Animals; Biological and medical sciences; Blood Platelets - virology; Genetic Engineering; Genetic Vectors - administration & dosage; Hematologic and hematopoietic diseases; Hematology; Injections, Intravenous; Macaca mulatta; Medical sciences; Platelet Count; Platelet diseases and coagulopathies; platelet survival; primates; thrombocytopenia; Thrombocytopenia - virology; toxicity; Thrombocytopenia; Animal model; Adenoviridae; Intravenous administration; Toxicity; Hemopathy; Survival; Virus; platelet survival; Vertebrata; Platelet; Mammalia; Animal; adenovirus; Primates; Complication
• ISSN: 0007-1048; 1365-2141
• DOI: 10.1046/j.1365-2141.2003.04719.x

A replication‐incompetent adenovirus vector was administered to rhesus macaques at 1, 3 and 6 × 1012 particles/kg doses to investigate its toxicity. Platelet count decrements of 28%, 82% and 90%, respectively, were observed, with corresponding platelet half‐lives of 69·0, 25·2 and 22·2 h (compared with 111 h in untreated animals). The platelet decline was equivalent for all three doses for 8 h, and platelet count recovery began as early as 8 h after infusion for low‐dose recipients, or as late as 24 h for the medium and high dose recipients. These observations suggest that thrombocytopenia is a saturable, reversible consumptive process.