New microsatellite loci for pomegranate, Punica granatum (Lythraceae)

• Premise of the study: A new set of pomegranate microsatellites was selected and characterized to assess the level of genetic diversity among cultivars and wild genotypes. • Methods and Results: Nine Simple Sequence Repeat (SSR) markers were obtained using the Microsatellite‐AFLP technique and were...

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Published inAmerican journal of botany Vol. 97; no. 7; pp. e58 - e60
Main Authors Currò, Sergio, Caruso, Marco, Distefano, Gaetano, Gentile, Alessandra, La Malfa, Stefano
Format Journal Article
LanguageEnglish
Published United States Botanical Society of America 01.07.2010
Botanical Society of America, Inc
Subjects
Botany
cultivar diversity
Cultivars
Flowers & plants
Genetic diversity
Genotype & phenotype
Lythraceae
microsatellite
Punica granatum L
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Summary:• Premise of the study: A new set of pomegranate microsatellites was selected and characterized to assess the level of genetic diversity among cultivars and wild genotypes. • Methods and Results: Nine Simple Sequence Repeat (SSR) markers were obtained using the Microsatellite‐AFLP technique and were successfully amplified in 34 genotypes belonging to Italian, Spanish, and Turkish germplasm collections. The number of alleles per locus ranged from 1 to 5, and the total number of alleles was 22. • Conclusions: Because only a few codominant markers are available for this species, the newly identified SSRs will facilitate genetic diversity studies, fingerprinting, and mapping. In addition, the 9 loci successfully amplified in P. granatum var. nana. No cross transferability was observed for Cuphea micropetala and Lagerstroemia indica (Lythraceae).
Bibliography:Lagerstroemia indica
Cuphea micropetala
The authors thank Yildiz Aka Kacar (Cukurova University, Adana, Turkey) and Anna Guglielmo (Dipartimento di Botanica, University of Catania, Italy) for providing the Turkish accessions and the samples of
used for microsatellite characterization.
and
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ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1000143