CELL FRACTIONATION ANALYSIS OF HUMAN CD8 GLYCOPROTEIN TRANSPORT BETWEEN ENDOPLASMIC RETICULUM, INTERMEDIATE COMPARTMENT AND GOLGI COMPLEX IN TISSUE CULTURED CELLS

• Published in: Cell biology international Vol. 23; no. 8; pp. 571 - 577
• Main Authors: Erra, Maria Carmen, Iodice, Luisa, Lotti, Lavinia Vittoria, Bonatti, Stefano
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.08.1999; Blackwell Publishing Ltd
• Subjects: Biological Transport - physiology; CD8 Antigens - analysis; CD8 Antigens - genetics; CD8 Antigens - metabolism; Cell Compartmentation - physiology; Cell Fractionation - methods; Cells, Cultured; endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Endoplasmic Reticulum - ultrastructure; Glycoproteins - analysis; Glycoproteins - genetics; Glycoproteins - metabolism; Glycosylation; Golgi Apparatus - metabolism; Golgi Apparatus - ultrastructure; Golgi complex; Humans; intermediate compartment; membrane traffic; Microscopy, Electron; Temperature; Transfection; intermediate compartment; Golgi complex; membrane traffic; endoplasmic reticulum
• ISSN: 1065-6995; 1095-8355
• DOI: 10.1006/cbir.1999.0420

We have set up an analytical cell fractionation procedure to dissect, by a non-morphological method, the anterograde transport of proteins from endoplasmic reticulum, intermediate compartment and Golgi complex in tissue cultured cells. Using this procedure after pulse-chase labelling of cells expressing human CD8 glycoprotein, we obtained results that: (1) support the view that the intermediate compartment is a distinct station in the export from the endoplasmic reticulum to the Golgi complex; and (2) strongly suggests that the O -glycosylation process starts after the intermediate compartment, presumably in thecis -Golgi complex.