Distinct endoplasmic reticulum stress responses are triggered during human liver transplantation
Injury due to cold ischaemia‐reperfusion (IR) represents a major cause of primary graft non‐function following human liver transplantation. This major cellular response translates into a dramatic decrease in intracellular ATP concentration during the ischaemic phase, thus sensitizing cells to reperf...
Saved in:
Published in | The Journal of pathology Vol. 207; no. 1; pp. 111 - 118 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
01.09.2005
Wiley |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Injury due to cold ischaemia‐reperfusion (IR) represents a major cause of primary graft non‐function following human liver transplantation. This major cellular response translates into a dramatic decrease in intracellular ATP concentration during the ischaemic phase, thus sensitizing cells to reperfusion shock. We postulated that IR‐induced cellular damage might cause alterations of the secretory pathway, particularly at the level of endoplasmic reticulum (ER) function. Under these circumstances, the ER triggers an adaptive response named the ‘unfolded protein response’ (UPR). In this study, we show that the expression of BiP, CHOP/GADD153 and GADD34, known to be induced specifically upon ER stress, are differentially affected upon IR, thus suggesting that distinct ER stress responses are activated during each phase of transplantation. With an approach combining semi‐quantitative RT‐PCR and immunoblotting using phospho‐specific antibodies, we show that the IRE‐1 pathway is activated upon early ischaemia and, in a second phase, upon early reperfusion. This occurs through the atypical splicing of XBP‐1 mRNA, its translation into a transcriptionally active XBP‐1 protein and the subsequent increase in EDEM mRNA expression, and may also contribute to the observed reperfusion‐induced activation of MAPK/SAPK. In contrast, we demonstrate that the PERK pathway, leading to inhibition of cap‐dependent translation, is mainly activated upon reperfusion, as shown by PERK and eIF2α phosphorylation. PERK activation is detected restrictively in sinusoidal endothelial cells and could contribute to the exaggerated sensivity of this liver cell type to IR injury. These results correlate well with the observed defect in protein secretion and suggest that the biphasic ER stress response may influence liver secretory functions and, as a consequence, condition liver transplantation outcomes. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. |
---|---|
Bibliography: | ArticleID:PATH1798 Fonds de la Recherche en Santé du Québec Canadian Institutes for Health Research - No. MOP53357 ark:/67375/WNG-PTTDX93W-C Fujisawa Inc. istex:984367D7D6E656B435AFFE3FAD4F798E50893C26 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-3417 1096-9896 |
DOI: | 10.1002/path.1798 |