Transforming Growth Factor β Downregulates Interleukin-1 (IL-1)-Induced IL-6 Production by Human Monocytes

• Published in: Blood Vol. 76; no. 12; pp. 2466 - 2469
• Main Authors: Musso, Tiziana, Espinoza-Delgado, Igor, Pulkki, Kari, Gusella, Gabriele L., Longo, Dan L., Varesio, Luigi
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 15.12.1990; The Americain Society of Hematology
• Subjects: Biological and medical sciences; Blotting, Northern; Cell physiology; Down-Regulation - drug effects; Fundamental and applied biological sciences. Psychology; Humans; Interleukin-1 - pharmacology; Interleukin-6 - genetics; Interleukin-6 - metabolism; Molecular and cellular biology; Monocytes - metabolism; Responses to growth factors, tumor promotors, other factors; RNA, Messenger - drug effects; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transforming Growth Factor beta - physiology; Human; Interleukin 6; Regulation(control); Monocyte; Transforming growth factor; Cytokine; Interleukin 1; Biosynthesis
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V76.12.2466.2466

We investigated the effects of transforming growth factor β (TGFβ) on the induction by interleukin-1β (IL-1β) of IL-6 in human monocytes. We found that IL-1β induced IL-6 messenger RNA expression in elutriated monocytes and IL-6 secretion in the supernatant. TGFβ did not induce IL-6. In contrast, TGFβ added to the culture inhibited, in a dose-dependent manner, the induction of IL-6 by IL-1 at the level of messenger RNA and bioactivity. These results show that IL-1β is able to stimulate IL-6 production by monocytes, TGFβ, by inhibiting this effect, may play an important role in regulating the IL-1-mediated components of the inflammatory response.