Diagnostic value of an enzyme-linked immunosorbent assay using the recombinant CT694 species-specific protein of Chlamydia trachomatis

• Published in: Journal of applied microbiology Vol. 107; no. 6; pp. 1875 - 1882
• Main Authors: Frikha-Gargouri, O, Gdoura, R, Znazen, A, Gargouri, J, Rebai, A, Hammami, A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.12.2009; Blackwell Publishing Ltd; Blackwell
• Subjects: Antigens, Bacterial - analysis; Antigens, Bacterial - genetics; bioinformatic tools; Biological and medical sciences; Child; Chlamydia Infections - diagnosis; Chlamydia Infections - microbiology; Chlamydia trachomatis; Chlamydia trachomatis - isolation & purification; Chlamydophila pneumoniae - isolation & purification; Cloning, Molecular; CT694 ELISA; CT694 hypothetical protein; Diagnosis, Differential; Enzyme-Linked Immunosorbent Assay - methods; expression vector; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Humans; Microbiology; microimmunofluorescence; Recombinant Proteins - analysis; Recombinant Proteins - genetics; Sensitivity and Specificity; CT694 hypothetical protein; Chlamydiaceae; Applied microbiology; CT694 ELISA; Chlamydia trachomatis; microimmunofluorescence; Gene expression; Protein; bioinformatic tools; Chlamydiales; Bacteria; Diagnosis; expression vector; Bioinformatics; ELISA assay; Vector
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2009.04365.x

To study the performance of the CT694 protein in relation to the microimmunofluorescence (MIF) and the pELISA tests for the serodiagnosis of Chlamydia trachomatis infections. The CT694 protein was produced as recombinant protein and was used as antigen in ELISA test for the detection of C. trachomatis IgG antibodies. The performance of the developed ELISA test was compared to the MIF test at two cut-off values of 16 and 64, and to the specific pELISA test using a panel of 342 sera. These sera were from children MIF C. trachomatis and Chlamydophila pneumoniae negative, patients MIF C. pneumoniae positive, patients MIF C. trachomatis positive, patients suspected to have chlamydial infections diagnosed by the Cobas Amplicor test, healthy blood donors and prostitutes. Our results indicate that the developed ELISA test has performed better compared with the MIF and the pELISA tests. The highest performance was obtained when comparing the developed ELISA test in relation to the pELISA, yielding an overall sensitivity and specificity of 85% and 87% respectively. The CT694 ELISA showed the best performance when compared to the species-specific pELISA test and may be used for the serodiagnosis of C. trachomatis infections. The CT694 ELISA test responds to the criteria of both sensitivity and specificity according to the MIF and pELISA tests and may be used for serodiagnosis of C. trachomatis infections.