RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR ACCELERATES REGENERATION AFTER T-2 TOXIN-INDUCED HEMOPOIETIC INJURY AND LESSENS LETHALITY IN MICE
The effects of rhG-CSF on T-2-induced leukopenia and lethal toxicity in mice were investigated. First, T-2 was administered by gavage to adult male mice at a dose of 3 mg/kg b. w. daily for 7 days, and rhG-CSF was given i. p. in daily dose of 10 or 30 μg/kg b. w./day, beginning on the 2nd day, for 5...
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Published in | Journal of toxicological sciences Vol. 18; no. 3; pp. 155 - 166 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Tokyo
The Japanese Society of Toxicology
1993
Japanese Society of Toxicology Japan Science and Technology Agency |
Subjects | |
Online Access | Get full text |
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Summary: | The effects of rhG-CSF on T-2-induced leukopenia and lethal toxicity in mice were investigated. First, T-2 was administered by gavage to adult male mice at a dose of 3 mg/kg b. w. daily for 7 days, and rhG-CSF was given i. p. in daily dose of 10 or 30 μg/kg b. w./day, beginning on the 2nd day, for 5 days. The peripheral WBC of mice receiving T-2 alone was decreased to one fourth of control counts, and bone marrow (BM) cell counts were also markedly diminished. The administration of rhG-CSF prevented those T-2-induced depressions. Histologically, the delation of the hematopoietic cells from BM and spleen of mice given T-2 was remarkably counteracted by administration of rhG-CSF. In the other experiment, rhG-CSF was injected i. p. for 5 days beginning on the next day of the 7-day T-2 administration. The recovery of WBC and BM cell counts was hastened by rhG-CSF reaching the control level in 6 days, and differential leukocyte analysis revealed an increase of neutrophils. Furthermore, simultaneous administration of rhG-CSF depressed the T-2-induced lethal toxicity, dose-dependently. The results revealed that rhG-CSF possesses a potent ability to protect T-2-induced leukopenia and lethality in mice, and it could be used as an antidote against T-2 and related trichothecene-induced acute intoxication. |
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ISSN: | 0388-1350 1880-3989 |
DOI: | 10.2131/jts.18.3_155 |