Testosterone Metabolism in the Isolated Perfused Human Foetal Liver

The isolated liver of a female pre‐viable foetus was perfused with [4‐14C]testosterone. 6β‐Hydroxy‐testosterone, 6β‐hydroxy‐androstenedione, 2β‐hydroxy‐testosterone and androstenedione could be isolated from the perfusion medium. These compounds have been characterized by their gas‐chromatographic b...

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Bibliographic Details
Published inEuropean journal of biochemistry Vol. 8; no. 2; pp. 284 - 286
Main Authors Demisch, K., Ammedick, U., Staib, W.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.03.1969
Subjects
17-Hydroxycorticosteroids - biosynthesis
17-Ketosteroids - biosynthesis
Acetates
Androstanes - biosynthesis
Carbon Isotopes
Chromatography, Gas
Crystallization
Ethers
Female
Fetus - metabolism
Humans
Liver - embryology
Liver - metabolism
Perfusion
Pregnancy
Sterols - biosynthesis
Testosterone - metabolism
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Summary:The isolated liver of a female pre‐viable foetus was perfused with [4‐14C]testosterone. 6β‐Hydroxy‐testosterone, 6β‐hydroxy‐androstenedione, 2β‐hydroxy‐testosterone and androstenedione could be isolated from the perfusion medium. These compounds have been characterized by their gas‐chromatographic behaviour on a SE‐30 and XE‐60 column, as free steroids, acetates and trimethylsilyl ethers. Furthermore 2β‐hydroxy‐testosterone as diacetate was crystallized to constant specific activity.
Bibliography:Note.
The following trivial names of steroids are used: aetiocholanolone = 3α‐hydroxy‐5β‐androstan‐17‐one; aetiocholandiol = 3α,17β‐dihydroxy‐5β‐androstan; androstenedione = androst‐4‐ene‐3,17‐dione; estrone = 3‐hydroxyestra‐1,3,5,(10)‐triene‐17‐one; estradiol = estra‐1,3,5(10)‐triene‐3,17β‐diol; estriol = estra‐1,3,5(10)‐triene‐3,16α,17β‐triol; 6β‐hydroxy‐testosterone = 6β,17β‐dihydroxy‐androst‐4‐ene‐3‐one; 6β‐hydroxy‐androstenedione = 6β‐hydroxyandrost‐4‐ene‐3,17‐dione; 2β‐hydroxy‐testosterone = 2β, 17β‐dihydroxy‐androst‐4‐ene‐3‐one; testosterone = 17β‐hydroxy‐androst‐4‐ene‐3‐one.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-2956
1432-1033
DOI:10.1111/j.1432-1033.1969.tb00525.x