Neuroproteomics in neurotrauma

• Published in: Mass spectrometry reviews Vol. 25; no. 3; pp. 380 - 408
• Main Authors: Ottens, Andrew K., Kobeissy, Firas H., Golden, Erin C., Zhang, Zhiqun, Haskins, William E., Chen, Su-Shing, Hayes, Ronald L., Wang, Kevin K.W., Denslow, Nancy D.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2006; Wiley
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Biomarkers; Brain Injuries - cerebrospinal fluid; Brain Injuries - genetics; Brain Injuries - metabolism; brain injury; Electrophoresis, Gel, Two-Dimensional; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; General aspects, investigation methods; Humans; Investigative techniques, diagnostic techniques (general aspects); mass spectrometry; Medical sciences; Nerve Tissue Proteins - biosynthesis; Nerve Tissue Proteins - cerebrospinal fluid; Nerve Tissue Proteins - genetics; Nervous system; neuroproteomics; Neuropsychological Tests; Neurosciences; neurotrauma; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; protein separations; Proteins; Proteome; Proteomics; Biological fluid; Animal model; Peptides; Biological marker; Review; Cerebrospinal fluid; Encephalon; neuroproteomics; brain injury; Clinical biology; Qualitative analysis; Bioinformatics; Quantitative analysis; Nervous system diseases; neurotrauma; Gel electrophoresis; Coupled method; Liquid chromatography; Protein; protein separations; Peptide fragment; Mass spectrometry MS/MS; Proteomics; Two dimensional electrophoresis; Head trauma; Mass spectrometry
• ISSN: 0277-7037; 1098-2787
• DOI: 10.1002/mas.20073

Neurotrauma in the form of traumatic brain injury (TBI) afflicts more Americans annually than Alzheimer's and Parkinson's disease combined, yet few researchers have used neuroproteomics to investigate the underlying complex molecular events that exacerbate TBI. Discussed in this review is the methodology needed to explore the neurotrauma proteome—from the types of samples used to the mass spectrometry identification and quantification techniques available. This neuroproteomics survey presents a framework for large‐scale protein research in neurotrauma, as applied for immediate TBI biomarker discovery and the far‐reaching systems biology understanding of how the brain responds to trauma. Ultimately, knowledge attained through neuroproteomics could lead to clinical diagnostics and therapeutics to lessen the burden of neurotrauma on society. © 2006 Wiley Periodicals, Inc., Mass Spec Rev 25:380–408, 2006