Expression of caveolin-3 immunoreactivities in the developing sciatic nerve of the rat

• Published in: Muscle & nerve Vol. 38; no. 2; pp. 1021 - 1026
• Main Authors: Lee, Ho J., Park, Choong H., Lee, Seong J., Park, Jin W., Choi, Jong H., Ryu, Gi H., Kwon, Bum S.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.08.2008; Wiley
• Subjects: Age Factors; Animals; Animals, Newborn; Biological and medical sciences; Caveolin 1 - metabolism; Caveolin 3 - genetics; Caveolin 3 - metabolism; caveolin-3; Development. Senescence. Regeneration. Transplantation; Diseases of striated muscles. Neuromuscular diseases; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental - physiology; immunohistochemistry; Indoles; Medical sciences; Myelin Basic Protein - metabolism; myelination; Neurofilament Proteins - metabolism; Neurology; peripheral nerve; Rats; Rats, Sprague-Dawley; RNA, Messenger - metabolism; Schwann cell; Sciatic Nerve - growth & development; Sciatic Nerve - metabolism; Vertebrates: nervous system and sense organs; Immunohistochemistry; Peripheral nerve; Nervous system diseases; Neuromuscular diseases; Rat; Myelin; Neuroglia; Rodentia; Reverse transcription; Muscular dystrophy; Protein; Genetic disease; Polymerase chain reaction; Vertebrata; Myelination; Mammalia; Postnatal; Schwann cell; caveolin-3; Muscle; Mutation; Sciatic nerve
• ISSN: 0148-639X; 1097-4598
• DOI: 10.1002/mus.20973

Caveolae are formed by the caveolin (CAV) family of proteins, CAV‐1, ‐2, and ‐3. CAV‐1 and ‐2 are co‐expressed in many cell types, whereas CAV‐3 is muscle‐specific and mutation of the CAV‐3 gene causes muscular dystrophy. CAV‐3 has also been detected in brain astroglial cells and in peripheral nerves along with CAV‐1. Therefore, we sought to determine whether CAV‐3 protein is expressed in developing peripheral nerves by using immunohistochemistry and reverse transcription–polymerase chain reaction (RT‐PCR). We found that CAV‐3 immunoreactivities (IRs) were localized in the myelin sheath during peripheral nerve development. CAV‐3 IRs were intense during the early postnatal stage, but decreased as the peripheral nerves matured at postnatal weeks 3–5. CAV‐3 mRNA expression was also markedly decreased during postnatal development. Because the expression pattern of CAV‐3 IRs was opposite that of CAV‐1 IRs, CAV‐1 and ‐3 might be involved in different phases of peripheral nerve myelination and play complementary roles in myelin maturation and peripheral nerve development. © 2008 Wiley Periodicals, Inc. Muscle Nerve 2008