Stimulation of B lymphocytes through surface Ig receptors induces LFA-1 and ICAM-1-dependent adhesion

• Published in: The Journal of immunology (1950) Vol. 146; no. 10; pp. 3273 - 3279
• Main Authors: Dang, LH, Rock, KL
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15.05.1991; American Association of Immunologists
• Subjects: Animals; Antigens, CD - physiology; B-Lymphocytes - immunology; Biological and medical sciences; Cell Adhesion; Cell Adhesion Molecules - analysis; Cell Adhesion Molecules - physiology; Cell Aggregation; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; Immunoglobulin M - immunology; Intercellular Adhesion Molecule-1; Lymphocyte Activation; Lymphocyte Function-Associated Antigen-1 - analysis; Lymphocyte Function-Associated Antigen-1 - physiology; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Mice; Mice, Inbred BALB C; Receptors, Antigen, B-Cell - metabolism; Receptors, Immunologic - physiology; Tetradecanoylphorbol Acetate - pharmacology; Immunoglobulins; Rodentia; Cell adhesion molecule; Cell cell interaction; Stimulation; B-Lymphocyte; Adhesion; Vertebrata; Membrane receptor; Mammalia; Mouse; T-Lymphocyte; Mechanism of action
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.146.10.3273

Engagement of the surface Ig receptor with anti-IgM antibodies stimulates murine B lymphocytes to markedly increase their expression of the cell adhesion molecules ICAM-1 and LFA-1. Stimulated B cells display increased homotypic adhesiveness and form spontaneous heterotypic conjugates with T lymphocytes. This latter T-B cell interaction is further enhanced if T cells have been previously activated with phorbol esters. In all cases, the formation of cell-cell conjugates is dependent on LFA-1-ICAM-1-mediated interactions as assessed in mAb blocking experiments. B lymphocytes stimulated with anti-IgM display a marked increase in binding to ICAM-1-transfected L cells. This cell-cell interaction is inhibited by anti-LFA-1 mAb binding to the B lymphocyte. Together, these results demonstrate that there is an induction of both ICAM-1 and LFA-1 on stimulated B cells and a corresponding increase in the adhesiveness of these cells. These findings suggest that Ag binding to the surface Ig receptor could prepare a B lymphocyte for subsequent interaction with a T lymphocyte. This provides insight into how efficient T-B collaboration may occur between very infrequent Ag-specific lymphocytes.