Chelation of Iron and Zinc by Protoporphyrin Catalyzed by Mitochondrial Preparations

1 Sonication, freezing and thawing, treatment of mitochondria with Tween‐Span surfactants and other procedures applied to isolate ferrochelatase from the liver mitochondria showed that the most active in releasing the enzyme was the combined action of Tween 20 at a final concentration of 15 mg/ml an...

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Published inEuropean journal of biochemistry Vol. 7; no. 4; pp. 583 - 587
Main Authors Mazanowska, A., Dancewicz, A. M., Malinowska, T., Kowalski, E.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.02.1969
Subjects
Animals
Chelating Agents
Chemical Phenomena
Chemical Precipitation
Chemistry
Chromatography
Chromatography, Gel
Freezing
Guinea Pigs
Iron
Lyases - isolation & purification
Mitochondria, Liver - enzymology
Porphyrins
Rats
Solubility
Surface-Active Agents
Swine
Tromethamine
Vibration
Zinc
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Summary:1 Sonication, freezing and thawing, treatment of mitochondria with Tween‐Span surfactants and other procedures applied to isolate ferrochelatase from the liver mitochondria showed that the most active in releasing the enzyme was the combined action of Tween 20 at a final concentration of 15 mg/ml and sonic irradiation on the mitochondrial suspensions, previously subjected to freezing and thawing. 2 A 20–40 fold purification of the enzyme was achieved by ammonium sulphate fractionation and chromatography on dextran gels of “solubilized” ferrochelatase. 3 Variations of the yield of the release of ferrochelatase from the mitochondria suggest that the localization of the enzyme within the mitochondrial structure is heterogeneous.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-2956
1432-1033
DOI:10.1111/j.1432-1033.1969.tb19646.x