Role of MicroRNA 126 in screening, diagnosis, and prognosis of diabetic patients in Egypt

• Published in: IUBMB life Vol. 68; no. 6; p. 452
• Main Authors: Rezk, Noha A, Sabbah, Norhan A, Saad, Mohamed S S
• Format: Journal Article
• Language: English
• Published: England 01.06.2016
• Subjects: Adult; Biomarkers - blood; Case-Control Studies; Diabetes Complications - genetics; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - diagnosis; Diabetes Mellitus, Type 2 - genetics; Egypt; Female; Glucose Intolerance - blood; Glucose Intolerance - genetics; Humans; Male; MicroRNAs - blood; Middle Aged; Prognosis; diabetes mellitus; complications; MicroRNA 126; IGT; qRT-PCR
• ISSN: 1521-6551
• DOI: 10.1002/iub.1502

MicroRNAs (miRNAs), family of non-coding small RNAs, play a vital role in the regulation of blood glucose level. We aimed to investigate the relation of serum miRNA-126 expression with impaired glucose tolerance as well as type 2 diabetes mellitus (T2DM) patients with and without complications. One hundred healthy controls, eighty-six patients with IGT, and one hundred patients with T2DM were recruited in this study. Serum miRNA-126 expression was assessed by quantitative real-time polymerase chain reaction. We found a significant decrease of serum miRNA-126 expression between IGT patients as well as diabetic patients when both compared with controls and between diabetic patients compared to IGT patients. A significant decrease of serum miRNA-126 expression was detected in diabetic patients with complications compared to those without evident complications especially those with diabetic macrovascular complications and diabetic retinopathy. Serum microRNA-126 expression could be a good marker for diagnosis of IGT and T2DM as well as for monitoring the outcomes of such disease. © 2016 IUBMB Life, 68(6):452-458, 2016.